Abstract:

To improve the thin layer chromatographic (TLC) identification standard of Yinqiao Powder in Chinese Pharmacopoeia, Yinqiao Powder samples were systematically extracted using different reagents to obtain different sample solutions. A series of TLC (sTLC) was used to systematically study the sample solutions, development conditions, and color development methods to identify 10 single herbs in Yinqiao Powder. Lonicerae Japonicae Flos, Forsythiae Fructus, Menthae Haplocalycis Herba, Arctii Fructus, Sojae Semen Praeparatum, Schizonepetae Spica, and Glycyrrhizae Radix Et Rhizoma were identified in Yinqiao Powder using sTLC. Arctii Fructus, Glycyrrhizae Radix Et Rhizoma, Sojae Semen Praeparatum, and Schizonepetae Spica can be dispersed on a thin-layer plate one time for common identification. In this study, the identifications of seven single herbs in Yinqiao Powder via sTLC can be used for identifying standard herbs. This approach shows good reproducibility, strong operability, and low detection cost. Furthermore, it can provide an experimental basis for the content supplement and quality standard improvement of Yinqiao Powder in Chinese Pharmacopoeia and facilitate a reference for the TLC identification of other Yinqiao Powder formulations.

Key words: Yinqiao Powder, series of thin layer chromatography, identification