Abstract:

We determined content variation of AstragalosideⅥ and AstragalosideⅡ in various probiotics fermented Radix Astragali with HPLCELSD. Determination conditions were chromatographic column of Agilent C18 column (250 mm×4.6 mm,5 μm）,mobile phase of acetonitrile and water mixture, a linear gradient elution, 1 mL/min flow rate. The contents of AstragalosideⅥ and AstragalosideⅡ in Radix Astragali were 0.476 2 mg/g and 0.050 3 mg/g. After fermentation with mixed probiotics, the contents of AstragalosideⅥ and AstragalosideⅡ were 0.257 8 mg/g and 0.109 4 mg/g.Results show that the content of AstragalosideⅡ increases through probiotics fermentation,but the content of AstragalosideⅥ decreases. Moreover, the contents of AstragalosideⅥ and AstragalosideⅡare different through various probiotics fermentation.

Key words: Astragaloside Ⅱ, probiotic fermentation, HPLC-ELSD, AstragalosideⅥ, Radix Astragali