二维高通量色谱分离制备海参来源真菌Epicoccum sp.的化学成分

doi:10.3976/j.issn.1002-4026.2015.04.003

Abstract

Abstract: We established a new method for separation and preparation of pimarane diterpenes from sea cucumberderived fungus Epicoccum sp.by twodimensional highthroughput chromatography.It was performed with MeOHH2O as solvent, flow rate of 40 mL/min, main separation detection wavelength of 254 nm and second separation detection wavelength of 220 nm.Pimarane diterpenes were isolated from 1.5 g ethyl acetate extract fromsea cucumberderived fungus Epicoccum sp.Their structureswere identified by spectroscopic data (1H NMR and MS).The method is simple and rapid, reduces irreversible sample adsorption,and provides a new approach for separation and preparation of pimarane diterpenes.

Key words: sea cucumberderived fungus, pimarane diterpenes, twodimensional highthroughput chromatography

CLC Number:

R284.2

QI Jun,XIA Xuekui,JIA Airong,LIU Xin,ZHANG Miansong,LIU Changheng. Separation and preparation of chemical components from sea cucumberderived fungus Epicoccum sp.by twodimensional highthroughput chromatography[J].SHANDONG SCIENCE, 2015, 28(4): 14-18.

References

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Separation and preparation of chemical components from sea cucumberderived fungus Epicoccum sp.by twodimensional highthroughput chromatography

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