J4 ›› 2011, Vol. 24 ›› Issue (4): 15-17.

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Content determination of total polysaccharides in leaves of Pyrus  betulaefolia Bge. from different processing methods

 WANG Shao-Jie1, ZHANG Ling-Ling2, XU Ling-Chuan3*, LIU Yu-Hong3, JIANG Hai-Qiang3   

  1. 1 Biology Institute, Shandong Academy of Sciences, Jinan 250014,China; 2 Hunan Provincial Baishaxi Tea Industry Co. Ltd., Anhua 413515, China; 3 Shandong University of Traditional Chinese Medicine, Jinan 250355, China
  • Online:2011-08-20 Published:2011-08-20

Abstract:

         We determined polysaccharides content of two manufactures from the leaves of Pyrus betulaefolia Bge. using phenolvitriol method.With the contrast, glucose, we measured their absorbency at the wavelength of 490 nm. A good linear relationship exists between the absorbency and the determined content in the range of 0.01968 ~0.0984 mg. Recovery experiment indicates that the average recovery rate is 99.02 % (n=5) and that RSD is 3.34 %. Determination results of total polysaccharide contents from different processes are 7.82% for that prepared by the traditional process of drying by sun and dewing in night and 5.27% for that prepared by sunbaked till to red after humidity accumulation. Experimental results show that the content of total polysaccharide in the leaves of P. betulaefolia Bge. is high. It can therefore be utilized as a total polysaccharide rich medical resource.

Key words: leaves of Pyrus betulaefolia Bge., total polysaccharide, method of phenolvitriol, content determination

CLC Number: 

  • R284.1