基于网络药理学、分子对接及动物实验探讨香附抗炎机制

doi:10.3976/j.issn.1002-4026.20260047

Abstract

Abstract: To investigate the anti-inflammatory activity and mechanism of Cyperi Rhizoma, network pharmacology was used to identify its active components and anti-inflammatory targets. Based on a zebrafish model and transcriptome sequencing, the anti-inflammatory activity and mechanism of Cyperi Rhizoma extracts were further validated. Subsequently, animal experiments and molecular docking were conducted to clarify the anti-inflammatory effects of core components and their potential targets. Network pharmacology identified core anti-inflammatory components (e.g., sugeonyl acetate and isorhamnetin) and targets (e.g., STAT3 and AKT1), involving the PI3K-Akt, MAPK, and other signaling pathways. Animal experiments confirmed that Cyperi Rhizoma extracts, as well as isorhamnetin, quercetin, and kaempferol, significantly inhibited the migration of immune cells in zebrafish, increased SOD activity, and reduced the production of NO and ROS. These effects were further enhanced following vinegar processing (P < 0.01). Transcriptome analysis indicated that the anti-inflammatory mechanism of Cyperi Rhizoma is closely associated with regulation of the ferroptosis pathway, significantly affecting the expression of ferroptosis-related genes (slc40a1, fth1, gpx4a/b, lpcat3, pcbp2) and inflammation-related genes (il8, il0, akt1, stat3) (P < 0.01 or P < 0.05). α-Cyperone, cyperenone, isorhamnetin, quercetin, and kaempferol significantly inhibited immune cell migration in zebrafish (P < 0.01), with flavonoid components showing stronger binding affinity toward LPCAT3, STAT3, and FTH1. Cyperi Rhizoma may exert its anti-inflammatory effects by acting on the core targets LPCAT3, STAT3, and FTH1 through synergistic regulation of multiple components, thereby inhibiting ferroptosis, alleviating oxidative stress, and ultimately reducing inflammation.

Key words: Cyperi Rhizoma, anti-inflammatory; zebrafish, network pharmacology, transcriptome, molecular docking, ferroptosis

CLC Number:

R285

SHAO Shuqin FAN Wenshuai WANG Zilong LIU Kechun ZHANG Shanshan. Study on the anti-inflammatory mechanisms of Cyperi Rhizoma based on network pharmacology, molecular docking, and animal experiments[J].Shandong Science, 0, (): 1-.

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Study on the anti-inflammatory mechanisms of Cyperi Rhizoma based on network pharmacology, molecular docking, and animal experiments

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