胶原蛋白神经酰胺脂质体的制备及质量评价

doi:10.3976/j.issn.1002-4026.20230144

Abstract

Abstract:

In this study, egg yolk lecithin, cholesterol,and ceramide were used as film-forming materials to prepare collagen ceramide liposomes (CO-CS) by the thin film dispersion method. To optimize the process, encapsulation efficiency was used as the response value, and it was evaluated by influencing factors such as drug-lipid ratio, film-material ratio, ultrasonic time, and hydration time. Results showed that encapsulation efficiency reached 90.73% when the drug-lipid ratio, film-material ratio, and ultrasonic time were 1:16, 4.3:1, and 8.5 min, respectively. Under these conditions, the particle size, polymer dispersity index (PDI), and zeta potential of CO-CS were(206.63±2.06) nm, 0.187±0.010, and (34.90±0.82) mV, respectively. After 60 days of storage at room temperature in the dark, the encapsulation efficiency was 87.2 %. Furthermore, the particle size, PDI, and zeta potential of CO-CS changed to (223.70±1.85) nm, 0.174±0.013, and (33.51±2.10) mV, respectively. In summary, CO-CS produced in this study revealed high encapsulation efficiency and good stability, and the preparation process is reasonable and feasible.

Key words: collagen, ceramide liposome, response surface, encapsulation efficiency

CLC Number:

TQ658.2

TANG Chunhui, ZHANG Zhenbo, WU Dongqin, HUAN Fei, ZHANG Jingshu, WANG Yubang. Preparation and quality evaluation of collagen ceramide liposomes[J].Shandong Science, 2024, 37(3): 18-26.

Figures/Tables 11

Table 1

Table 2

Fig.1

Table 3

Table 4

Variance analysis of regression model and its effect on encapsulation efficiency"

差异来源	平方和	自由度	均方	F值	P值	显著性
模型	780.18	9	0.314 65	91.60	< 0.000 1	**
A	42.64	1	0.281 25	45.06	0.000 3	**
B	11.14	1	0.061 25	11.77	0.011 0	*
C	142.38	1	0.005	150.45	< 0.000 1	**
AB	0.10	1	0.16	0.11	0.751 8	不显著
AC	1.29	1	0.062 5	1.36	0.281 5	不显著
BC	16.48	1	0.002 5	17.42	0.004 2	**
A²	197.81	1	0.725 158	209.03	< 0.000 1	**
B²	163.25	1	0.114 632	172.50	< 0.000 1	**
C²	145.79	1	1.227 789	154.05	< 0.000 1	**
残差	6.62	7	0.030 071
失拟项	4.31	3	0.067 5	2.49	0.199 5	不显著
纯误差	2.31	4	0.002
总和	786.80	16
R²					0.991 6
$R a d j 2$					0.980 8
$R p r e d 2$					0.907 7

Table 4

Fig.2

Table 5

Fig.3

Fig.4

Fig.5

Table 6

References 18

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处方组成	用量	处方组成	用量
胶原蛋白原液	1.3 mL	神经酰胺	0.02 g
蛋黄卵磷脂	0.1 g	无水乙醇	5 mL
胆固醇	0.02 g	1%吐温-80	0.5 mL

因素	水平
因素	-1	0	1
药脂比 A	1:20	1:16	1:12
超声时间B/min	5	10	15
膜材比 C	3:1	5:1	7:1

实验号	药脂比A	超声时间B/min	膜材比C	包封率Y/ %	实验号	药脂比A	超声时间B/min	膜材比C	包封率Y/ %
1	1:12	5	5:1	75.55	10	1:16	15	7:1	73.95
2	1:20	5	5:1	80.39	11	1:16	5	3:1	84.46
3	1:12	15	5:1	72.34	12	1:16	15	3:1	79.21
4	1:20	15	5:1	76.54	13	1:16	10	5:1	90.15
5	1:12	10	7:1	70.98	14	1:16	10	5:1	89.45
6	1:20	10	7:1	74.56	15	1:16	10	5:1	89.66
7	1:12	10	3:1	77.40	16	1:16	10	5:1	89.04
8	1:20	10	3:1	83.25	17	1:16	10	5:1	88.13
9	1:16	5	7:1	71.08

实验次数	粒径/nm	PDI	电位/mV	包封率/%
1	206.63	0.185	-34.1	88.54
2	207.33	0.172	-35.5	92.82
3	203.87	0.185	-33.9	91.17
4	205.80	0.195	-34.7	88.57
5	209.50	0.196	-35.7	92.53
均数±标准差	206.63±2.06	0.187±0.010	-34.9±0.8	90.73±2.08

时间/d	外观性状	包封率/%	粒径/nm	PDI	Zeta 电位/mV
7	白色混悬液,流动性良好	90.83±0.40	210.80±0.51	0.172±0.006	-34.50±0.38
14	白色混悬液,流动性良好	90.32±2.71	213.02±3.63	0.180±0.028	-35.44±0.64
21	白色混悬液,流动性良好	89.64±1.01	218.61±1.69	0.169±0.009	-34.60±1.14
28	白色混悬液,流动性良好	89.08±0.99	220.14±1.52	0.171±0.012	-34.72±1.59
60	白色混悬液,流动性良好	87.20±1.09	223.70±1.85	0.174±0.013	-33.51±2.10

Preparation and quality evaluation of collagen ceramide liposomes

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