不同价态的阴离子在DNA凝聚效应中的抑制作用

doi:10.3976/j.issn.1002-4026.2020.05.015

Abstract

Abstract: To quantitatively study the inhibitory effect of anions on DNA condensation and charge neutralization, the effect of anions of various valencies on cation-mediated DNA condensation was studied using dynamic light scattering and atomic force microscopy (ATM). Dynamic light scattering experiments showed that anions reduced the electrophoretic mobility of DNA in the presence of cations, indicating that charge neutralization of DNA was inhibited. The higher valence of anions, the stronger was the inhibition of electrophoretic mobility. Different anions exerted the same inhibitory effect and trend on DNA charge neutralization at the same concentration of various cations. Meanwhile, the morphological changes in DNA condensation as observed by AFM led to the same conclusion. At the same cation concentration, highvalent cations showed more potent inhibition on DNA condensation than lowvalent cations, which make the DNA aggregation morphology more loose. These observations further confirmed that anions have a weakening effect on cationmediated DNA condensation.

Key words: DNA condensation, charge inversion, anion, cation, inhibitory effect

CLC Number:

LI Cong-cong, YANG Guang-can, WANG Yan-wei. Inhibition of DNA condensation by anions of various valencies[J].Shandong Science, 2020, 33(5): 119-126.

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Inhibition of DNA condensation by anions of various valencies

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