Abstract:

The contents of water, ash, leachate and (R, S)-goitrin in Isatidis Radix from 7 regions such as Inner Mongolia, Shaanxi, Gansu, Hebei, Henan, Shandong, and Heilongjiang were determined and compared. The results showed that the water content of Isatidis Radix in different producing areas ranged from 8.86% to 11.72%, and the lowest in Shaanxi.The total ash content was between 3.55% and 7.00%, and the content of ash in Shandong was the lowest.The acidinsoluble ash content was 0.14% ~ 0.62%, the content of acid-insoluble ash in Shandong was the lowest.The content of alcohol-soluble extract was from 29.99% to 43.71%, the content of Isatidis Radix extract in Shaanxi was the highest.The result of HPLC content measurement showed that the content of (R, S)-goitrin was 0.444 ~ 1.042 mg/g, (R, S)-goitrin in the Isatidis Radix from Henan produced was the highest content. The water, ash, leachate and (R, S)-Goitrin content of Isatidis Radix in each producing area met the standards. The experimental results have certain guiding significance for agricultural production and clinical drug use.

Key words: content determination, Isatidis Radix, (R, S)-goitrin