Abstract:

A HPLC method for the determination of paeoniflorin in tonic semifluid extract of ten ingredients was established. The HPLC analysis was performed on Kromasil C18 column (4.6 mm×250 mm, 5 μm), acetonitrilewater (17∶83, V/V) as the mobile phase, and the column temperature was 30 ℃, injection volume was 10 μL, the detective wavelength was at 230 nm, at a flow rate of 1.0 mL／min. Results revealed that paeoniflorin at the content of 0.083 6～0.627 0 μg showed good linearity（r= 0.999 8），the recovery rate was 98.98%～100.47%, RSD was 0.65% (n=6). This method is highly specific, sensitive, reproducible, and simple, which can be used for the quality control of this preparation.

Key words: HPLC, tonic semifluid extract of ten ingredients, paeoniflorin, content determination