Abstract:

A HPLC method for simultaneous determination of the contents of six main bioactive constituents (protocatechuic acid, chlorogenic acid, vanillic acid, caffeic acid, mangiferin, and rutin) in the Pyrrosia petiolosa (Christ) Ching was presented. The analysis was performed on SinoChrom ODS-BP C18（4.6 mm × 150 mm，5 μm）column. The six components were separated with gradient elution using mobile phase composed of 0.1% acetic acid (A)acetonitrile (B) at a flow rate of 1.0 mL/min. The UV detection wavelength was set at 320 nm. The column temperature was kept at 25℃. Six ingredients showed good linear relationship (R≥0.999 2, n=5), with relative standard deviation values less than 5%. Results reveal that this method is rapid, simple, and accurate, to provide experimental foundation for the quantitative detection and quality control of Medical Pyrrosia petiolosa (Christ) Ching.

Key words: HPLC, determination, ingredient, Pyrrosia petiolosa (Christ) Ching