铜、汞和铅对斑马鱼仔鱼肝脏发育的影响

doi:10.3976/j.issn.1002-4026.2016.05.009

Abstract

Abstract:

We addressed the impact of three typical heavy metal pollutants (copper chloride, mercury chloride and lead acetate) on liver growth of zebrafish larvae (72~144 hpf) with a liver green fluorescence transgenic zebrafish T3 (lfabp:EGFP) as a model. Results show that the survival rates of all groups are all more than 95%. CuCl₂ and HgCl₂ of 1 μmol/L can all cause abnormality of swimbladder growth or inflation. The three heavy metal compounds can all significantly reduce liver fluorescence area and integral optical density. CuCl₂ has more powerful inhibition capability for liver growth than Pb（Ac）₂ and HgCl₂ under identical exposure concentration. However, HgCl₂ of 0.01 μmol/L can inhibit liver growth. Inhibition capability of lfabp expression of CuCl2 and Pb（Ac）₂ is greater that of HgCl₂. Moreover, Pb（Ac）₂ of 0.01 μmol/L can inhibit the expression of lfabp. Results show that the three heavy metal compounds can all significantly inhibit liver growth. Transgenic zebrafish larvae can serve as priority model for detection of environmental contaminants caused liver growth toxicity.

Key words: lead, mercury, zebrafish, liver growth, copper, larvae

CLC Number:

R965.1

HAN Jian, PENG Weibing, HAN Liwen, HE Qiuxia, ZHANG Yun, SUN Chen, WANG Rongchun, CHU Jie, LIU Kechun. Impact of Cu,Hg and Pb on liver growth of zebrafish larvae[J].SHANDONG SCIENCE, 2016, 29(5): 54-59.

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Impact of Cu,Hg and Pb on liver growth of zebrafish larvae

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