复方葛根片对衰老大鼠胸主动脉损伤的保护作用

doi:10.3976/j.issn.1002-4026.2016.05.010

Abstract

Abstract:

We divided rats into model group and normal group, and high, middle and low dose group of compound puerarin tablet to investigate the impact of compound puerarin tablet on D-galactose induced serum homocysteine (Hcy) level and protective effect on thoracic aorta injury of aged rats. We employed subcutaneous D-galactose injection to establish rats aging model, AU5800 automatic biochemical analyzer to detect Hcy content in rats serum, and SPSS16 to analyze Hcy content and weight difference of each group. We also performed HE staining to rats thoracic aorta and observed its pathological changes with a light microscope. Results show that significant difference (P<0.01) exists in Hcy level and weight of model group and normal group, each dose group and model group. Different dose groups have different protective effects on thoracic aortic vessels, especially for the high dose group having best protective effect. We discover that compound puerarin tablets can reduce Hcy levels in D-galactose induced old rats serum and have protective effects on vascular injury of thoracic aorta.

Key words: D-galactose, Homocysteine, aged rat

CLC Number:

R285.5

LI Yunjiang，SUN Lin，BI Yunsheng，ZHANG Shuyu，YU Yanli. Protective effect of compound puerarin tablet on thoracic aorta injury of aged rats[J].SHANDONG SCIENCE, 2016, 29(5): 60-64.

References

［1］王洋.益气活血中药通过外膜干预血管衰老及血管衰老的中医证型特点研究［D］.北京：中国中医科学院,2012 .
［2］ UNGVARI Z, KALEY G, de CABO R, et al. Mechanisms of vascular aging: New perspectives［J］. J Gerontol A Biol Sci Med Sci, 2010,65(10): 10281041.
［3］WALDＤＳ，LAW M,MORRIS J K.Homocysteine and cardiovascular disease:Evidence on causality from a metanalysis［J］.BMJ,2002,325(7374):12021206.
［4］秦红兵,杨朝晔,范忆江,等.D半乳糖诱导衰老小鼠模型的建立和评价［J］.中国组织工程研究与临床康复,2009,13（7）：12751278.
［5］VIGEN R,MADDOX T M,ALLEN L A.Aging of the United States population: Impact on heart failure［J］.Curr Heart Fail Rep,2012,9( 4) : 369374.
［6］鲍溪荷,徐将,叶山东,等.甲钴胺对 T2DM 患者血 Hcy 水平和尿 MCP1、TGFβ1排泄的影响［J］.中华疾病控制杂志,2014,11（18）：10951098.
［7］杨安宁,王磊,周龙霞,等.ABCA1、ACAT1 DNA 甲基化在 Hcy 致 THP1单核源性泡沫细胞胆固醇流出中作用研究［J］.中国药理学通报,2014,30（3）：340344.
［8］BOUSHEY C J,BERESFORD S A A,OMENN G S,etal.A quantitative assessment of plasms homocysteine as a risk factor for vascular disease［J］.JAMA,1995,274(13):1049 1057.
［9］孙卫华,张晓梅,李晓丽,等.Hcy、Cys C 和 VEGF 与 2型糖尿病微血管病变的关系研究［J］.中华全科医学,2014.12（10）：15471549.
［10］LOSCALZO J.Homocysteine trialscldar outcomes for complex reasons［J］. N EngI J Med,2006,354,(15):16291632.
［11］付伟光,齐振勇,李爱军,等.HCY、HsCRP、LP（a）及 DD 联合检测在冠心病病程中的应用研究［J］.现代预防医学,2014,41（1）:122125.
［12］XU D，NEVILLE R，FINKEL T．Homocysteine accelerates endothelial cell senescence ［J］． FEBS Lett，2000，470 ( 1 ) :2024.
［13］JIA S J,LAI Y Q,ZHAO M,et al.Homocysteineinduced hypermethylation of DDAH2 promoter contributes to apoptosis of endothelial cells［J］.Die Pharmazie,2013,68(4):282286．
［14］于燕莉，范云飞，毕云生，等.冠舒滴丸对衰老模型大鼠脑组织的保护作用［J］.中国药房，2013,24（47）：44394441.
［15］GONZALEZ C D，LEE M S，MARCHETTI P，et al． The emerging role of autophagy in the pathophysiology of diabetes mellitus［J］．Autophagy，2011, 7 ( 1 ) :211.

Metrics

Comments

Recommended 0

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0), which permits third parties to freely share (i.e., copy and redistribute the material in any medium or format) and adapt (i.e., remix, transform, or build upon the material) the articles published in this journal, provided that appropriate credit is given, a link to the license is provided, and any changes made are indicated. The material may not be used for commercial purposes. For details of the CC BY-NC 4.0 license, please visit: https://creativecommons.org/licenses/by-nc/4.0

[1]	LIU Kechun, WANG Yongcheng, ZANG Xiaohan, XIA Qing, ZHANG Yun, ZHANG Shanshan, SUN Chen. Advancements in network pharmacology and zebrafish modeling for studying traditional Chinese medicine’s effective substances and mechanisms of action [J]. Shandong Science, 2024, 37(2): 29-35.
[2]	WU Yonghao, XIE Hebing, HOU Hairong, WANG Rongchun, CHEN Xiqiang. Protective effect of Feirepuqing Powder on hypoxia-induced sports injury in zebrafish model by inhibiting Hif1α [J]. Shandong Science, 2024, 37(1): 32-38.
[3]	ZHAO Xin, HUANG Biyun, WU Dan, ZHANG Mei. The mechanism of Guishaozhenxian Tablet in the treatment of temporal lobe epilepsy based on network pharmacology and molecular docking [J]. Shandong Science, 2023, 36(3): 27-37.
[4]	LIU Wenwen, LI Changgeng, KONG Na, LIU Shuang, DONG Hongjing. Study on the mechanism of Shiweiyipi Granules based on network pharmacology and molecular docking [J]. Shandong Science, 2023, 36(2): 33-40.
[5]	DENG Zhi-peng, ZHANG Xiao, TIAN Hai-tao. Identification of metabolites of isochlorogenic acid A in rats based on UHPLC-Q-Exactive Orbitrap MS [J]. Shandong Science, 2022, 35(6): 58-64.
[6]	LIANG Meng-meng, SHAO Xin-xin, CHEN Cong, ZHANG Song, ZHANG Guang-zhong, LI Jia. The basis and mechanism of Rosae Rugosae Flos antidepressants based on network pharmacology [J]. Shandong Science, 2022, 35(4): 38-48.
[7]	DU Hai-tao, WANG Ping, LI Na, HAN Li, DING Jie, HU Ya-nan. The mechanism underlying Fagopyri Dibotryis Rhizoma's action against respiratory syncytial virus using network pharmacology [J]. Shandong Science, 2022, 35(4): 28-37.
[8]	LI Xiao-li,YING Tian-hao,TANG Yi-di,ZHU Xuan,SUN Meng-meng,YU Tao,ZHAO Jia-ning,ZHANG Lei-ming. Potential mechanism of Mahuangjiazhu Decoction in the treatment of rheumatoid arthritis based on network pharmacology combined with molecular docking technology [J]. Shandong Science, 2022, 35(3): 17-26.
[9]	CAO Ming-chen,PENG Xiao-ju,HUANG Xian-ju,KE Cai-hua,WANG Chuan,XIN Zhao-yang,YU Zhen-ying,PAN Dong-qi,CUI Meng-na. Mechanism of Fuzheng Tiaoli Decoction based on network pharmacology and its effect on S180 tumor-bearing mice [J]. Shandong Science, 2022, 35(1): 45-55.
[10]	LIU Hao, HAN Chang-sheng, CHI Xian-su, YANG Zhen-xiao, WANG Xiang-yu, WANG Xin, MA Ke. Therapeutic effects and mechanism of action of Suanzaoren decoction, a classical prescription, on p-chlorophenylalanine-induced insomnia in rats [J]. Shandong Science, 2021, 34(6): 32-40.
[11]	ZHANG Xian, LI Yue. Analysis of flavonoids in mulberry leaves from different production areas [J]. Shandong Science, 2021, 34(4): 34-39.
[12]	ZOU Hong-yuan, ZHU Cheng-yue, ZHANG Shan-shan, KONG Rui-qi, ZHANG Yun, LIU Ke-chun. Protective effect of peony seed oil microcapsule powder on isoniazid-induced developmental toxicity in zebrafish [J]. Shandong Science, 2021, 34(4): 45-51.
[13]	CAO Ming-chen, XU Long, XIN Zhao-yang, FANG Ming-xiang, XING Xiao-min, CHEN Xiao, WANG Chuan, WEI Xin-xin, REN Wei. Network pharmacology study of Fritillariae Thunbergii Bulbus - Trichosanthis Fructus compatibility for treating chronic obstructive pulmonary disease based on the BATMAN-TCM online analysis platform [J]. Shandong Science, 2021, 34(1): 10-20.
[14]	WANG Jun, SU Ben-zheng, JIANG Hai-qiang, SUN Yu, YANG Yu-han, CUI Ning, YU Zong-yuan. Exploration of the mechanisms of Cinnamomi Cortex in the treatment of diabetes based on network pharmacology [J]. Shandong Science, 2020, 33(6): 44-54.
[15]	DING Xiao-yan, ZU Long-hui, WANG Bian-li. Application of partial least squares regression analysis in the study of the antibacterial spectrum-effect relationship and mathematical model of Scutellariae Radix [J]. Shandong Science, 2020, 33(4): 18-23.

Protective effect of compound puerarin tablet on thoracic aorta injury of aged rats

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0