基于BATMAN-TCM在线分析平台的浙贝母-瓜蒌配伍治疗慢性阻塞性肺病的网络药理学研究

doi:10.3976/j.issn.1002-4026.2021.01.002

Abstract

Abstract: Based on the bioinformatics analysis tool for molecular mechanism of traditional Chinese medicine (BATMAN-TCM), this study revealed the molecular mechanism of action of Fritillariae Thunbergii Bulbus-Trichosanthis Fructus (FTB-TF) for treating chronic obstructive pulmonary diseases (COPD). The BATMAN-TCM was adopted to predict the potential targets of FTB-TF, whereas the GeneCards database was used to search the targets of COPD. Cytoscape 3.7.1 software was used to construct a compound-disease-target interaction network and protein-protein interaction (PPI) network to conduct gene ontology (GO) enrichment analysis and pathway enrichment analysis based on the Kyoto encyclopedia of genes and genomes (KEGG). A total of 21 types of active ingredients were screened from FTB-TF, and the compound-disease-target network was constructed. The PPI network of FTB-TF targets was established based on STRING, and it included 131 nodes and 236 edges. Examples of the key nodes included ANXA1, EDN1, HDAC1, RXRA, ADRA2A, OXT, and RXRB. The GO enrichment analysis mainly involved ion-gated channel activity, substrate-specific channel activity, passive transmembrane transporter activity, extracellular ligand-gated ion channel activity, neurotransmitter receptor activity, and ligand-gated ion channel activity. The KEGG pathway analysis mostly involved neuroactive ligand-receptor interaction, cAMP signaling pathway, calcium signaling pathway, cholinergic synapse, retrograde endocannabinoid signaling, serotonergic synapse, and dopaminergic synapse. The results suggested that ANXA1 and HDAC1, for instance, were the key targets of FTB-TF. The signaling pathways primarily involved neuroreceptor pathways such as acetylcholine, serotonin, and dopamine. These findings reflected the overall characteristics of the Chinese medicine compound prescription, thereby providing new clues for further research on the molecular mechanism of action of FTB-TF.

Key words: Fritillariae Thunbergii Bulbus-Trichosanthis Fructus, BATMAN-TCM, GO, KEGG, network pharmacology;chronic obstructive pulmonary disease

CLC Number:

R285.5

CAO Ming-chen, XU Long, XIN Zhao-yang, FANG Ming-xiang, XING Xiao-min, CHEN Xiao, WANG Chuan, WEI Xin-xin, REN Wei. Network pharmacology study of Fritillariae Thunbergii Bulbus - Trichosanthis Fructus compatibility for treating chronic obstructive pulmonary disease based on the BATMAN-TCM online analysis platform[J].Shandong Science, 2021, 34(1): 10-20.

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Network pharmacology study of Fritillariae Thunbergii Bulbus - Trichosanthis Fructus compatibility for treating chronic obstructive pulmonary disease based on the BATMAN-TCM online analysis platform

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