Abstract:

      We extracted 27 traditional Chinese herbs with petroleum ether, ethyl acetate and water, and then determined the antiangiogenic activity of the extracts with a transgenic zebrafish model (VEGFR2:GFP). We further calculated the intersegmental  vessels（ISV）of the zebrafish embryos and the inhibitory rate after 24 h treatment with different extracts. We eventually tested the activity of some active extracts with chick embryo chorioallantoic membrane (CAM). Results show that antiangiogenic activity is exhibited for water extract of Rhizoma Ligustici Chuanxiong,ethyl acetate extract of Radix Paeoniae alba, water extract of Stroma Shiraiae,water extract and ethyl acetate extract of Euonymus alatus,water extract of Boswellia carterii Birdw. and ethyl acetate extract of Folium Isatidis.Angiogenesis of CAM is significantly inhibited for water extracts of Rhizoma Ligustici Chuanxiong,water extract and ethyl acetate extract of Euonymus alatus and water extract of Boswellia carterii Birdw. We discover that 7 extracts of traditional Chinese herbs can inhibit the sprout and growth of ISV of a transgenic zebrafish, which is relevant to the expression of vascular endothelial grouth factor (VEGFR2).

Key words: traditional Chinese herb, zebrafish, angiogenesis