响应面法优化金银花中有机酸类成分提取工艺

doi:10.3976/j.issn.1002-4026.20240053

Abstract

Abstract:

The extraction rate of organic acid components was used as an indicator to investigate the effects of extraction process parameters, namely, extraction temperature, extraction time, solid-liquid ratio, and particle size on the extraction results using the water extraction method. A response surface model was established using a Box-Behnken design to optimize the extraction process parameters for organic acid components from Lonicera japonica Thunb. with temperature, time, and solid-liquid ratio as factors. Results show that the optimal extraction process for organic acid components from Lonicera japonica Thunb. comprise an extraction temperature of 72 ℃, an extraction time of 90 min, and a solid-liquid ratio of 1 g∶40 mL. Under these conditions, the extraction rate of organic acid components can reach 5.16% with a relative error of 0.96% compared to the theoretical value.

Key words: Lonicera japonica Thunb., organic acids, extraction, the response surface methodology

CLC Number:

R284.2

LI Wanying, WANG Haiyan, WANG Huicheng, ZHANG Peng, Fang Lei. Optimization of the extraction process for organic acid components obtained from Lonicera japonica Thunb. using response surface methodology[J].Shandong Science, 2025, 38(4): 1-7.

Figures/Tables 10

Fig.1

Fig.2

Fig.3

Fig.4

Table 1

Table 2

Table 3

Variance analysis results"

方差来源	平方和	自由度	均方	F值	P值	显著性
模型	0.68	9	0.075	12.71	0.001 5	**
X₁	0.099	1	0.099	16.71	0.004 6	**
X₂	0.070	1	0.070	11.87	0.010 8	*
X₃	0.25	1	0.25	41.35	0.000 4	**
X₁X₂	0.078	1	0.078	13.23	0.008 3	**
X₁X₃	2.5×10^-5	1	2.5×10^-5	4.219×10^-3	0.950 0
X₂X₃	0.011	1	0.011	1.86	0.214 8
$X 1 2$	0.15	1	0.15	24.98	0.001 6	**
$X 2 2$	5.921×10^-3	1	5.921×10^-3	1.00	0.350 8
$X 3 2$	0.011	1	0.011	1.78	0.224 3
残差	0.041	7	5.925×10^-3
失拟项	0.023	3	7.758×10^-3	1.71	0.302 9
纯误差	0.018	4	4.550×10^-3
总离差	0.72	16
相关系数(R²)	0.942 3

Table 3

Fig.5

Fig.6

Fig.7

References 13

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水平	因素
水平	时间/min	温度/℃	料液比/(g·mL^-1)
-1	30	70	1:30
0	60	80	1:35
1	90	90	1:40

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Optimization of the extraction process for organic acid components obtained from Lonicera japonica Thunb. using response surface methodology

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试验编号	水平			有机酸提取率Y /%
试验编号	时间X₁	温度X₂	料液比X₃	有机酸提取率Y /%
1	0	-1	1	5.18
2	0	1	-1	4.97
3	0	0	0	5.11
4	0	0	0	5.07
5	0	0	0	5.02
6	1	0	-1	4.80
7	-1	0	1	4.94
8	1	0	1	5.20
9	1	-1	0	4.98
10	0	1	1	5.17
11	0	0	0	5.18
12	0	0	0	5.17
13	1	1	0	4.98
14	-1	0	-1	4.55
15	-1	-1	0	4.51
16	-1	1	0	5.07
17	0	-1	-1	4.77