胶原蛋白神经酰胺脂质体的制备及质量评价

doi:10.3976/j.issn.1002-4026.20230144

Abstract

Abstract:

In this study, egg yolk lecithin, cholesterol,and ceramide were used as film-forming materials to prepare collagen ceramide liposomes (CO-CS) by the thin film dispersion method. To optimize the process, encapsulation efficiency was used as the response value, and it was evaluated by influencing factors such as drug-lipid ratio, film-material ratio, ultrasonic time, and hydration time. Results showed that encapsulation efficiency reached 90.73% when the drug-lipid ratio, film-material ratio, and ultrasonic time were 1:16, 4.3:1, and 8.5 min, respectively. Under these conditions, the particle size, polymer dispersity index (PDI), and zeta potential of CO-CS were(206.63±2.06) nm, 0.187±0.010, and (34.90±0.82) mV, respectively. After 60 days of storage at room temperature in the dark, the encapsulation efficiency was 87.2 %. Furthermore, the particle size, PDI, and zeta potential of CO-CS changed to (223.70±1.85) nm, 0.174±0.013, and (33.51±2.10) mV, respectively. In summary, CO-CS produced in this study revealed high encapsulation efficiency and good stability, and the preparation process is reasonable and feasible.

Key words: collagen, ceramide liposome, response surface, encapsulation efficiency

CLC Number:

TQ658.2

TANG Chunhui, ZHANG Zhenbo, WU Dongqin, HUAN Fei, ZHANG Jingshu, WANG Yubang. Preparation and quality evaluation of collagen ceramide liposomes[J].Shandong Science, 2024, 37(3): 18-26.

Figures/Tables 11

Table 1

Table 2

Fig.1

Table 3

Table 4

Variance analysis of regression model and its effect on encapsulation efficiency"

差异来源	平方和	自由度	均方	F值	P值	显著性
模型	780.18	9	0.314 65	91.60	< 0.000 1	**
A	42.64	1	0.281 25	45.06	0.000 3	**
B	11.14	1	0.061 25	11.77	0.011 0	*
C	142.38	1	0.005	150.45	< 0.000 1	**
AB	0.10	1	0.16	0.11	0.751 8	不显著
AC	1.29	1	0.062 5	1.36	0.281 5	不显著
BC	16.48	1	0.002 5	17.42	0.004 2	**
A²	197.81	1	0.725 158	209.03	< 0.000 1	**
B²	163.25	1	0.114 632	172.50	< 0.000 1	**
C²	145.79	1	1.227 789	154.05	< 0.000 1	**
残差	6.62	7	0.030 071
失拟项	4.31	3	0.067 5	2.49	0.199 5	不显著
纯误差	2.31	4	0.002
总和	786.80	16
R²					0.991 6
$R a d j 2$					0.980 8
$R p r e d 2$					0.907 7

Table 4

Fig.2

Table 5

Fig.3

Fig.4

Fig.5

Table 6

References 18

[1]	GU L S, SHAN T T, MA Y X, et al. Novel biomedical applications of crosslinked collagen[J]. Trends in Biotechnology, 2019, 37(5): 464-491. DOI: 10.1016/j.tibtech.2018.10.007. pmid: 30447877
[2]	AL-NIMRY S, DAYAH A A, HASAN I, et al. Cosmetic, biomedical and pharmaceutical applications of fish gelatin/hydrolysates[J]. Marine Drugs, 2021, 19(3): 145. DOI: 10.3390/md19030145.
[3]	张燕. 鹿皮胶原蛋白多肽脂质体水凝胶的制备及其在小鼠光老化中的保护作用[D]. 长春: 吉林大学, 2022.
[4]	KOVÁČIK A, KOPEČNÁ M, VÁVROVÁ K. Permeation enhancers in transdermal drug delivery: benefits and limitations[J]. Expert Opinion on Drug Delivery, 2020, 17(2): 145-155. DOI: 10.1080/17425247.2020.1713087.
[5]	孙娅楠, 赵静, 李超华, 等. 二次谐波结合双光子荧光成像方法观察人源胶原蛋白透皮吸收情况[J]. 激光生物学报, 2017, 26(1): 24.
[6]	SUN Y N, LI L S, MA S H, et al. In vivo visualization of collagen transdermal absorption by second-harmonic generation and two-photon excited fluorescence microscopy[J]. Frontiersin Chemistry, 2022, 10: 925931. DOI: 10.3389/fchem.2022.925931.
[7]	ALBASH R, YOUSRY C, AL-MAHALLAWI A M, et al. Utilization of PEGylated cerosomes for effective topical delivery of fenticonazole nitrate: in-vitro characterization, statistical optimization, and in-vivo assessment[J]. Drug Delivery, 2021, 28(1): 1-9. DOI: 10.1080/10717544.2020.1859000.
[8]	王玉真, 高爽, 孙越, 等. 菊苣酸脂质体制备工艺研究[J]. 山东科学, 2020, 33(1): 8-15. DOI: 10.3976/j.issn.1002-4026.2020.01.002.
[9]	马永强, 王姿淇, 黎晨晨, 等. 番茄红素肝靶向脂质体制备工艺研究及肝靶向性评价[J]. 中国食品添加剂, 2023, 34(4): 106-115. DOI: 10.19804/j.issn1006-2513.2023.04.013.
[10]	苏林豫, 朱效素, 张蓬, 等. 新型蛋白降解靶向嵌合体LC-2脂质体的制备和表征[J]. 中国医药工业杂志, 2022, 53(5): 679-687. DOI: 10.16522/j.cnki.cjph.2022.05.013.
[11]	龚频, 方文静, 赵文婧, 等. 灵芝多糖脂质体制备工艺[J]. 食用菌学报, 2022, 29(5): 101-108. DOI: 10.16488/j.cnki.1005-9873.2022.05.011.
[12]	CHEN W T, WU H T, CHANG I C, et al. Preparation of curcumin-loaded liposome with high bioavailability by a novel method of high pressure processing[J]. Chemistry and Physics of Lipids, 2022, 244: 105191. DOI: 10.1016/j.chemphyslip.2022.105191.
[13]	张婷, 温鹤迪, 宋敬一, 等. 基于乙醇注入-高压均质的蛋清肽脂质体制备及体内外缓释效果[J]. 食品科学, 2021, 42(13): 79-86. DOI: 10.7506/spkx1002-6630-20200624-331.
[14]	RAMLAN N, ZUBAIRI S I, MASKAT M Y. Response surface optimisation of polydimethylsiloxane (PDMS) on borosilicate glass and stainless steel (SS316) to increase hydrophobicity[J]. Molecules, 2022, 27(11): 3388. DOI: 10.3390/molecules27113388.
[15]	马永强, 隋佳琦, 黎晨晨, 等. 响应面法优化高顺式虾青素的制备工艺研究[J]. 食品与发酵工业, 2023: 1-15.
[16]	国家药典委员会. 中华人民共和国药典四部2020年版[M]. 北京: 中国医药科技出版社, 2020.
[17]	GULDIKEN B, GIBIS M, BOYACIOGLU D, et al. Physical and chemical stability of anthocyanin-rich black carrot extract-loaded liposomes during storage[J]. Food Research International, 2018, 108: 491-497. DOI: 10.1016/j.foodres.2018.03.071. pmid: 29735084
[18]	ZHAO L S, TEMELLI F. Preparation of anthocyanin-loaded liposomes using an improved supercritical carbon dioxide method[J]. Innovative Food Science & Emerging Technologies, 2017, 39: 119-128. DOI: 10.1016/j.ifset.2016.11.013.

Metrics

Comments

Recommended 0

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0), which permits third parties to freely share (i.e., copy and redistribute the material in any medium or format) and adapt (i.e., remix, transform, or build upon the material) the articles published in this journal, provided that appropriate credit is given, a link to the license is provided, and any changes made are indicated. The material may not be used for commercial purposes. For details of the CC BY-NC 4.0 license, please visit: https://creativecommons.org/licenses/by-nc/4.0

处方组成	用量	处方组成	用量
胶原蛋白原液	1.3 mL	神经酰胺	0.02 g
蛋黄卵磷脂	0.1 g	无水乙醇	5 mL
胆固醇	0.02 g	1%吐温-80	0.5 mL

因素	水平
因素	-1	0	1
药脂比 A	1:20	1:16	1:12
超声时间B/min	5	10	15
膜材比 C	3:1	5:1	7:1

实验号	药脂比A	超声时间B/min	膜材比C	包封率Y/ %	实验号	药脂比A	超声时间B/min	膜材比C	包封率Y/ %
1	1:12	5	5:1	75.55	10	1:16	15	7:1	73.95
2	1:20	5	5:1	80.39	11	1:16	5	3:1	84.46
3	1:12	15	5:1	72.34	12	1:16	15	3:1	79.21
4	1:20	15	5:1	76.54	13	1:16	10	5:1	90.15
5	1:12	10	7:1	70.98	14	1:16	10	5:1	89.45
6	1:20	10	7:1	74.56	15	1:16	10	5:1	89.66
7	1:12	10	3:1	77.40	16	1:16	10	5:1	89.04
8	1:20	10	3:1	83.25	17	1:16	10	5:1	88.13
9	1:16	5	7:1	71.08

实验次数	粒径/nm	PDI	电位/mV	包封率/%
1	206.63	0.185	-34.1	88.54
2	207.33	0.172	-35.5	92.82
3	203.87	0.185	-33.9	91.17
4	205.80	0.195	-34.7	88.57
5	209.50	0.196	-35.7	92.53
均数±标准差	206.63±2.06	0.187±0.010	-34.9±0.8	90.73±2.08

时间/d	外观性状	包封率/%	粒径/nm	PDI	Zeta 电位/mV
7	白色混悬液,流动性良好	90.83±0.40	210.80±0.51	0.172±0.006	-34.50±0.38
14	白色混悬液,流动性良好	90.32±2.71	213.02±3.63	0.180±0.028	-35.44±0.64
21	白色混悬液,流动性良好	89.64±1.01	218.61±1.69	0.169±0.009	-34.60±1.14
28	白色混悬液,流动性良好	89.08±0.99	220.14±1.52	0.171±0.012	-34.72±1.59
60	白色混悬液,流动性良好	87.20±1.09	223.70±1.85	0.174±0.013	-33.51±2.10

Preparation and quality evaluation of collagen ceramide liposomes

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 11

References 18

Related Articles 13

Metrics

Comments

Recommended 0

[1]	WANG Haiyan, YU Ping, WANG Xin, YU Hongxia, ZHOU Jie, FANG Lei. Optimization of enzyme-water continuous extraction of ginsenosides from fibrous roots of American ginseng using response surface methodology [J]. Shandong Science, 2025, 38(5): 10-21.
[2]	LI Wanying, WANG Haiyan, WANG Huicheng, ZHANG Peng, Fang Lei. Optimization of the extraction process for organic acid components obtained from Lonicera japonica Thunb. using response surface methodology [J]. Shandong Science, 2025, 38(4): 1-7.
[3]	MIAO Xuzhong, LI Xiufen, GAO Yanyan, ZHANG Xue, ZHANG Xiqing, QIAO Luqin. Optimization of preparation of persimmon peel polysaccharide chromium using response surface methodology [J]. Shandong Science, 2024, 37(2): 127-134.
[4]	GUO Tian-tian, FANG Zhao-ying, LIU Zhen-yuan, ZHANG Wei, BI Yan-yan, SUN Yan, GUAN Yong-xia. Optimization of extraction process and antioxidant activity of Angelica sinensis volatile oil [J]. Shandong Science, 2022, 35(6): 16-23.
[5]	BI Yan-yan, MA Qing-wen, XU Hui, LIU Jian, LONG Rui, TANG Wei-ming, GUO Tian-tian, HAO Da-wei. Optimization of the column chromatographic purification of total flavonoids extracted from Ginkgo biloba using Box-Behnken design and response surface method [J]. Shandong Science, 2022, 35(6): 8-15.
[6]	JIA Quan, HU Li-min, REN Feng, ZHANG Jian-li, TIAN Hong-nian, LIU Shu-bin. Optimization of the freeze concentration process of ceftazidime mother liquor based on the response surface method [J]. Shandong Science, 2021, 34(5): 121-129.
[7]	CHENG Jun-shan, LI He, YANG Ji-zong, PAN Li-hong, GU Xiao-fei, ZHANG Miao-jing. Application of solvent-free crystallization technology in the production of vitamin B₁₂ [J]. Shandong Science, 2021, 34(2): 96-103.
[8]	SU Ming, YUAN Min, SUN Xiao-tong, SUI Zai-yun. Determination of paeonol and ganoderan content in Compound Paeonol Nanoemulsion [J]. Shandong Science, 2020, 33(6): 27-33.
[9]	DU Qing-wei, YUAN Min , LIANG Rui-xue. Optimization of the molding process for Linbitong granules using central composite design-response surface methodology [J]. Shandong Science, 2020, 33(4): 7-12.
[10]	WU Jia-jin, BIAN Zhi-jie, ZHU Yue, ZHANG Xue-tao, PANG Li-dan, GAO Peng. Investigation on preparation process of pigskin collagen peptide-zinc chelate [J]. Shandong Science, 2020, 33(3): 53-61.
[11]	WANG Yu-zhen, GAO Shuang, SUN Yue, ZHANG Li, BO Fu-min, FENG An-jie, LI Ling-jun. Preparation process of chicoric acid liposome [J]. Shandong Science, 2020, 33(1): 8-15.
[12]	LIU Xiao-mei, LI Yuan-yuan, WANG Sheng-guang, MA Qing-lin, JIANG Shan, GAO Peng. Preparation and hepatoprotective effect of selenium chelated by corn peptide [J]. Shandong Science, 2019, 32(1): 29-37.
[13]	LI Yuan-yuan,JIANG Yan-ling， ZHOU Hong-lei，ZHANG Ling, CAI Mei-chao， SUN Xiao-lin, CHU Xin-hong. Optimization of alcohol extraction techniques of total flavonoids from Bidens bipinnata L. stems using response surface methodology [J]. SHANDONG SCIENCE, 2017, 30(5): 21-27.