Abstract:

The redox reaction will occur when aflatoxin contacts with its oxidase, which can produce hydrogen peroxide and other products. In this research, the method of enzyme electrode biosensors was used to immobilize aflatoxin oxidase on cellulose acetate carrier membrane and  prepare electrochemical enzyme electrode. Aflatoxin oxidase electrode was installed on the SBA flow injection analysis instrument to detect the content of aflatoxin. The results showed that the toxin enzyme electrode had good response characteristics to aflatoxin, the precision (RSD) was up to 1.20% (n=10), the linear range was 0 ~100 μg/L (R2 =0.999 6), and the recovery rate was 96%~102.4%. Therefore, the enzyme electrode analysis method established in this study can be used for the detection of aflatoxin B1 in maize.

Key words: enzyme electrode, aflatoxin, corn, aflatoxin oxidase