HPLC法同时测定忍冬的花、叶中木犀草苷和绿原酸含量

doi:10.3976/j.issn.1002-4026.2013.04.005

J4 ›› 2013, Vol. 26 ›› Issue (4): 20-23.doi: 10.3976/j.issn.1002-4026.2013.04.005

• Traditional Chinese medicine and natural active products • Previous Articles Next Articles

Determination of luteoloside and chlorogenic acid in flowers and leaves from Lonicera japonica Thunb. with HPLC

1. Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, China; 2. School of Life Sciences, Shandong Normal University, Jinan 250014, China; 3. Shandong Yate Ecotech Co. Ltd., Linyi 266071, China

Received:2012-10-03 Online:2013-08-20 Published:2013-08-20

Abstract

Abstract:

We constructed a determination approach for the contents of luteoloside and chlorogenic acid in flowers and leaves from Lonicera japonica Thunb. with HPLC. Their compounds were completely separated under the conditions of Shimpack ODS chromatographic column, gradient elution of Acetionitrile and 0.2% methanoic acid, flow rate of 1.0 mL/min and detection wavelength of 350 nm. Results show that luteoloside content in the leaves of Yate, Italy and Jiufengyihao is 5.856, 2.454 and 4.633 mg·g^-1, much higher than that in their flowers.

Key words: Lonicera japonica Thunb., flower, leaf, luteoloside, chlorogenic acid, HPLC

CLC Number:

R284.1

SHI Xin-Gang, SHU Xi-Kai, LIU Wei, LIU Feng, LI Sheng-Bo, WANG Xiao, WANG Dai-Jie. Determination of luteoloside and chlorogenic acid in flowers and leaves from Lonicera japonica Thunb. with HPLC[J].J4, 2013, 26(4): 20-23.

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Determination of luteoloside and chlorogenic acid in flowers and leaves from Lonicera japonica Thunb. with HPLC

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