Abstract: Based on the network pharmacology method to predict the action mechanism of Salviae Miltiorrhizae Radix Et Rhizoma-Bupleuri Radix on the treatment of alcoholic liver disease. Screen the effective active ingredients of Salviae Miltiorrhizae Radix Et Rhizoma and Bupleuri Radix and the corresponding target proteins through the database, and predict and screen the target of Salviae Miltiorrhizae Radix Et Rhizoma-Bupleuri Radix for the treatment of alcoholic liver disease. Use Cytoscape 3.7.2 software to construct a drug-component-target-disease network diagram. Map the key target protein-protein interaction (PPI) network. Metascape database was used to conduct gene ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis for effective targets. Sixty-five Salviae Miltiorrhizae Radix Et Rhizoma compounds with 162 effective targets and 17 Bupleuri Radix compounds with 251 effective targets were screened. Through screening, 161 intersecting targets of Salviae Miltiorrhizae Radix Et Rhizoma and Bupleuri Radix for alcoholic liver disease were obtained. PPI network analysis revealed that AKT1, IL6, TP53, VEGFA, TNF, CASP3, and EGF may be the key targets of Salviae Miltiorrhizae Radix Et Rhizoma and Bupleuri Radix for the treatment of alcoholic liver disease. GO annotation analysis involved signaling pathways such as reactions to inorganic substances, reactions to toxic substances, membrane rafts, dendrites, transcription factor binding, and protein domain-specific binding. KEGG pathway analysis involved tumor signaling pathways, fluid shear stress and atherosclerosis, proteoglycans in cancer, and AGE-RAGE signaling pathways in diabetic complications. The key targets of Salviae Miltiorrhizae Radix Et Rhizoma and Bupleuri Radix medicine for the treatment of alcoholic liver disease as well as the biological processes and signaling pathways involved were preliminarily explored, and it was found that the effect is multi-target and multi-pathway.

Key words: Salviae Miltiorrhizae Radix Et Rhizoma, Bupleuri Radix, alcoholic liver disease, network pharmacology, mechanism of action