J4 ›› 2012, Vol. 25 ›› Issue (4): 27-31.doi: 10.3976/j.issn.1002-4026.2012.04.007

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HS-SPME-GC-MS analysis of volatile components in fresh and dried leaves of  Artemisia argyi

 WANG Li1,2, ZHAO Xian-En1, WANG Xiao1, GENG Yan-Ling1, LIU Jian-Hua1, LIU Wei1   

  1. 1.Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, China; 2.School of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China
  • Received:2012-04-08 Online:2012-08-20 Published:2012-08-20

Abstract:

        We analyzed volatile components in fresh and dried leaves of Artemisia argyi Levl. et Vant. with headspace solid phase microextraction ( SPME) and GC-MS. We also investigated the impacts of different SPME fibers and extraction temperature on the extracts. Results demonstrate that 65 μm PDMS /DVB fibre and temperature of 60 ℃ exhibit good extraction effect.We employed peak area normalization method to determine the volatile components. Fifty-eight compounds are identified in the fresh and dried leaves of Artemisia argyi, which include alkanes,alkenes and alcohols,and whose area exceeds 90% of the total peak area. Results also show that fresh and dried leaves of Artemisia argyi not only contain identical volatile compounds such as Camphene, 1-Octen-3-ol, alpha Phellandrene, Benzene, 1-methyl-2 (1-methylethyl) Eucalyptol and Caryophyllene, but also contain their own unique volatile compounds. This shows that their volatile  components  change after dryness.

Key words: fresh leaves of , Artemisia argyi, dried leaves of Artemisia argyi, volatile components, headspace solid phase microextraction (HS-SPME), GC-MS

CLC Number: 

  • O652.62