Abstract:

We employed headspace solid phase microextraction (SPME) and GC-MS to analyze the volatile components of four different species of Chaenomeles speciosa Nakai. We also investigated the effects of different SPME fibers and extraction positions on extraction results. Experimental results show that 65 μm PDMS/DVB fiber exhibits better extraction result.The pericarp and flesh of different positions have not significant influence on their volatile components. We further employed peak area normalization method to determine the relative content of each volatile component. We identified 60 volatile components, which mainly included esters, alkanes and alkenes whose total content exceeded 90%. The varieties and relative contents of the volatile components of these Chaenomeles speciosa Nakai were different. This method is rapid and accurate, so it can provide reference for further development and utilization of Chaenomeles speciosa Nakai resources.

Key words: Chaenomeles speciosa Nakai, volatile components, headspace solid phase microextraction (HS-SPME), GC-MS