桂枝HPLC指纹图谱研究

doi:10.3976/j.issn.1002-4026.2013.05.010

Abstract

Abstract:

We constructed high performance liquid chromatography (HPLC) fingerprint for Ramulus Cinnamomi to provide a new method for its quality evaluation and identification. We analyzed thirteen batches of Ramulus Cinnamomi with C18 column (Agilent Eclipse XDBC18 column), gradient elution solvent system including water and acetonitrile, flow rate of 0.8 mL/min, column temperature of 25℃ and detection wavelength of 285 nm. The method had better precision, repetition and stability. Thirteen shared peaks and four specific peaks exist in the thirteen batches of fingerprints. Similarity analysis shows that the quality of Guangdong and Guangxi samples has slight difference. HPLC fingerprinting will be a potential identification and quality evaluation tool for Ramulus Cinnamomi.

Key words: Ramulus Cinnamomi, HPLC, fingerprint

CLC Number:

R284.1

ZHAO Heng-Qiang, GENG Yan-Ling, WU Hong-Wei, DUAN Wen-Juan, YANG Hong-Jun, LIU Jian-Hua, WANG Xiao. HPLC fingerprint of Ramulus Cinnamomi[J].J4, 2013, 26(5): 47-51.

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Open Access This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0), which permits third parties to freely share (i.e., copy and redistribute the material in any medium or format) and adapt (i.e., remix, transform, or build upon the material) the articles published in this journal, provided that appropriate credit is given, a link to the license is provided, and any changes made are indicated. The material may not be used for commercial purposes. For details of the CC BY-NC 4.0 license, please visit: https://creativecommons.org/licenses/by-nc/4.0

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HPLC fingerprint of Ramulus Cinnamomi

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