菝葜多糖分离纯化工艺研究

doi:10.3976/j.issn.1002-4026.2021.06.001

Abstract

Abstract:

The purpose of this study is to optimize the polysaccharide purification technique from Smilacis Chinae Rhizoma. With the purity, paste rate, adsorption rate, and other parameters as indicators, the optimal alcohol precipitation process and macroporous resin purification technique were determined by investigating the alcohol precipitation temperature, alcohol precipitation time, macroporous resin type, and other factors. The optimum alcohol precipitation process is as follows. Take 1.0 g/mL solution and add ethanol to make the alcohol content reach 80%. Then, perform alcohol precipitation once and leave it at 25 ℃ for 12 h. Finally, pass it through a suction filter to obtain alcohol precipitate and dry it at 70 ℃. Optimum purification technique is as follows. Load 1 BV sample (2.0 mg/mL) and pass it through AB-8 resin at the rate of 2 BV/h, followed by elution through 3 BV pure water at the rate of 3 BV/h. The results show that the optimized process is stable and reliable and can be used for purifying.

Key words: Smilacis Chinae Rhizoma, polysaccharide, alcohol precipitation, macroporous resin

CLC Number:

R284.2

Ming MEI,Jin ZHOU,Yi ZHANG,Liang-peng MA,Ming YANG,Song HU. Isolation and purification of polysaccharide from Smilacis Chinae Rhizoma[J].Shandong Science, 2021, 34(6): 1-8.

Figures/Tables 13

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References 19

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时间/h	多糖纯度/%	多糖出膏率/%
6 12 24	46.21 51.54 52.12	1.42 2.12 2.14
48	52.14	2.15

提取液生药质量浓度/(g·mL^-1)	多糖纯度/%	多糖出膏率/%
0.5 0.8 1.0	43.95 46.81 51.89	1.97 2.09 2.15
1.2	51.81	2.07

乙醇体积分数/%	多糖纯度/%	多糖出膏率/%
60 70 80	32.92 49.12 51.23	1.87 1.98 2.15
90	51.25	2.13

项目		多糖纯度/%	多糖出膏率/%
编号	1 2 3	51.51 52.47 51.32	2.13 2.11 2.12
均值		51.77	2.12

上样量/BV	吸附率/%
1	69.2
2	65.5
3	59.2
4	55.3
5	49.8

Isolation and purification of polysaccharide from Smilacis Chinae Rhizoma

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洗脱溶剂	吸光度
纯水	0.676
10%乙醇	0.663
15%乙醇	0.427
30%乙醇	0.332
40%乙醇	0.142

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项目		多糖纯度/%	多糖得率/%
编号	1	60.43	1.01
	2	60.51	1.03
	3	60.49	1.06
均值		60.48	1.03