基于PI3K/AKT/Gsk-3β通路研究山药多糖对运动疲劳小鼠的影响

doi:10.3976/j.issn.1002-4026.20240034

Abstract

Abstract:

This study aimed to investigate the antifatigue effects and mechanisms of Dioscorea opposita Thunb. polysaccharides (DTPs) on exercise-related fatigue in mice. In a mouse model of exercise fatigue where mice were subjected to exhaustive swimming and fatigue rotarod, changes in exhaustive swimming duration, body weight, fatigue metabolite accumulation, activity levels of related enzymes, liver tissue structure, and muscle glycogen (MuG) and liver glycogen (LG) contents were assessed. The results demonstrated that DTPs significantly prolonged the exhaustive swimming duration and reduced body weight loss, hepatic damage, and serum lactic acid (LD), blood urea nitrogen (BUN), and lactate dehydrogenase (LDH) levels in the mice. DTPs also significantly enhanced superoxide dismutase (SOD) activity and MuG and LG contents and significantly reduced aspartate aminotransferase (AST) levels. Additionally, in mice administered DTPs, the mRNA expression of phosphoinositide 3-kinase (PI3K) was significantly upregulated, whereas that of protein kinase B (AKT) and glycogen synthase kinase-3 (Gsk-3β) was significantly downregulated in skeletal muscle. Taken together, DTPs can reduce metabolite accumulation and ameliorate exercise fatigue by regulating the PI3K/AKT/Gsk-3β pathway to promote glycogen synthesis.

Key words: Dioscorea opposita Thunb. polysaccharides, anti-exercise fatigue, exhaustive swimming, PI3K/AKT/Gsk-3β pathway

CLC Number:

ZENG Ting, YANG Ran, LI Qingjun, GONG Yanan, WANG Shuzhen, LIU Qian. Effects of Dioscorea opposita Thunb. polysacchariades on exercise-related fatigue in mice through the PI3K/AKT/Gsk-3β pathway[J].Shandong Science, 2024, 37(6): 51-58.

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References 28

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基因	上游(5'-3')	下游(5'-3')
PI3K	GGGAGCAGCAACCGAAACAAAG	CCACTACGGAGCAGGCATAGC
AKT	GCCACGCTACTTCCTCCTCAAG	GACTCTCGCTGATCCACATCCTG
Gsk-3β	TGTGTTGGCTGAATTGTTGCTAGG	AATTTGCTCCCTTGTTGGTGTTCC
β-actin	GCGCAAGTACTCTGTGTGGA	CATCGTACTCCTGCTTGCTG

组别	肝细胞水样性变性	肝细胞坏死	免疫细胞浸润
CG	少量	无	无
MG	弥漫性	少量	淋巴细胞
DTP-L	弥漫性	无	无
DTP-H	大量	无	无
PG	多量	无	无

Effects of Dioscorea opposita Thunb. polysacchariades on exercise-related fatigue in mice through the PI3K/AKT/Gsk-3β pathway

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