Abstract:

The optimum liquid medium of white  Hypsizigus marmoreus can provide the fundamental data for its extensive cultivation. We optimized fermentation medium with L₉(3⁴) orthogonal test, the main ingredients of glucose, yeast powder, KH₂PO₄ and MgSO₄, and exopolysaccharides content and mycelial biomass as test indexes. We also separated mycelia by extraction and filtering, dried and weighted them, and calculated their biomass. Phenol sulfuric acid was then employed to determine the content of exopolysaccharides. Weighted evaluation of exopolysaccharides content and mycelial biomass shows that the optimum liquid medium is composed of glucose of 3%, yeast powder of 0.15%, KH₂PO₄ of 0.30% and MgSO₄ of 0.20%.

Key words: white Hypsizigus marmoreus, mycelial biomass, exopolysaccharides, orthogonal test, weighted mark method