正交试验法优选黄芩配方颗粒的水提工艺

doi:10.3976/j.issn.1002-4026.2017.06.004

SHANDONG SCIENCE ›› 2017, Vol. 30 ›› Issue (6): 22-28.doi: 10.3976/j.issn.1002-4026.2017.06.004

• Traditional Chinese medicine and natural active products • Previous Articles Next Articles

Optimization of the water extraction conditions of Huangqin dispensing granules by orthogonal test

HAO Cui^1,2, MU Yan²,ZHAO Heng-qiang²,CUI Li², LIU Wei², LIU Feng-qin^3*

1.Shandong University of Traditional Chinese Medicine, Jinan 250355,China;2.Shandong Provincial Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center，Jinan 250014, China; 3.Shandong Qianfoshan Hospital, Jinan 250014, China

Received:2017-05-27 Online:2017-12-20 Published:2017-12-20

Abstract

Abstract:

For the optimization of the water extraction conditions of Huangqin dispensing granules, orthogonal test method was used to investigate the effects of 3 factors, including different water volumes, extraction frequency and extraction duration, on the total content of baicalin, wogonoside, baicalein and wogonin and the extract rate. Meanwhile, the methodology of the HPLC determination was verified. As a result, under the experiment conditions, the importance order was as follows: extraction duration,extraction frequency,water volume. And extraction durations at different level were significant different (P＜0.05). The experiment proved that the suitable extraction condition was that Huangqin slices were added 10 times volume of water and extracted 3 times for 1 h each time. The extraction condition optimized in this paper was practical and stable with high extraction rate of active ingredients, which was suitable to be used in the preparation of Huangqin dispensing granules.

Key words: Huangqin dispensing granules, orthogonal test, water extraction conditions

CLC Number:

R284.2

HAO Cui, MU Yan,ZHAO Heng-qiang,CUI Li, LIU Wei, LIU Feng-qin. Optimization of the water extraction conditions of Huangqin dispensing granules by orthogonal test[J].SHANDONG SCIENCE, 2017, 30(6): 22-28.

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Optimization of the water extraction conditions of Huangqin dispensing granules by orthogonal test

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