Abstract: In this study, high performance liquid chromatography was performed to detect the content of six flavonoids in 22 batches of mulberry leaves purchased from different regions of the country. Precision, stability, repeatability, sample recovery rate, and flavonoid content were measured to determine the types and contents of flavonoids in mulberry leaves from different regions. The results showed that the relative standard deviation (RSD) of the chromatographic peak area detected by precision and stability tests were both <1%， this indicates that the detection instrument and operation meet the precision requirements. The amount of flavonoids considerably varied in mulberry leaves from different regions. Among them, the total amount of flavonoids in mulberry leaves collected from Bozhou, Anhui; Mianyang, Sichuan; and Yulin, Guangxi was high, followed by that in leaves collected from Shangqiu, Henan; Tongxiang, Zhejiang; Taixing, Jiangsu; and Xiangyang, Hubei. The total amount of flavonoids in mulberry leaves collected from Shaoyang, Hunan was the lowest. The results suggest that the content of flavonoids in mulberry leaves from different regions is different. The determination of the content of flavonoids in mulberry leaves via high performance liquid chromatography is easy to operate, stable, and repeatable. 

Key words: mulberry leaf, origin, flavonoids, content analysis；HPLC