Abstract: Based on the extraction and transfer rates of specnuezhenide,oleanolic acid,and ursolic acid as indicators,the best extraction process for Fructus Ligustri Lucidi was deduced and optimized using single-factor investigation and an orthogonal experimental design.Results revealed that the optimum extraction process involved the use of eight-fold 60% ethanol,3-fold extraction,and 3-hour duration per round of extraction.The transfer rates of specnuezhenide,oleanolic acid,and ursolic acid were 75.42%,93.86%,and 99.83% respectively.The optimized extraction process features desirable extraction effects and a simple procedure,which can be efficiently used for industrial production.

Key words: Fructus Ligustri Lucidi, specnuezhenide, oleanolic acid, ursolic acid, extraction process