Abstract:

A UPLC method for the determination of rutin, luteoloside, chlorogenic acid and quercetin from Salix songarica flower was established. The analysis was separated on ACQUITY UPLC BEH C18 (100 mm×2.1 mm, 1.7 μm) column, with acetonitrile (A) and water (containing 0.2% acetic acid, B), gradient elution. The flow rate was 0.29 mL/min and detected at 360 nm; the column temperature was 30 ℃. The linear range was 0.05~3.12 μg/mL (r=0.999 8), and the average recovery was 99.9%，with the RSD of 1.6% for rutin. The linear range was 0.03~9.00 μg/mL (r=0.999 5), and the average recovery was 98.8%，with the RSD of 0.8% for luteolin. The linear range was 0.01~5.00 μg/mL (r=0.999 7), and the average recovery was 97.6%，with the RSD of 1.7% for quercetin. The linear range was 0.001~0.05 μg/mL (r=0.999 8), and the average recovery was 99.0%，with the RSD of 2.0% for chlorogenic acid. This method was accurate and reliable, which can be regarded as a reference to control the quality of rutin, luteolin, quercetin and chlorogenic acid in the Salix songarica flower.

Key words: galuteolin, quercetin, Salix songarica flower, chlorogenic acid, rutin