Abstract:

A rapid ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method was developed for the simultaneous analysis of wogonin, 4dicaffeoylquinic acid and chrysin-7-O-Beta-D-glucoronide in rats plasma. Apiin was selected as the internal standard. The analysis was carried out on an ACQUITY UPLC HSS T3 column (2.1 mm×100 mm, 1.8 μm), with acetonitrile and 0.1% formic acid aqueous solution, gradient elution. The flow rate was 0.2 mL·min^-1. Linear responses were obtained for wogonin, 4-dicaffeoylquinic acid and chrysin-7-O-Beta-D-glucoronide ranging from 1 to 1 000 ng·mL^-1. The correlation coefficients were better than 0.99. Both recovery rates and matrix effects met the requirement of biological sample analysis. The established UHPLC-MS/MS quantitative analysis method shows high sensitivity, accuracy and satisfying recovery, and can be utilized to determine the pharmacokinetic data of wogonin, 4-dicaffeoylquinic acid and chrysin-7-O-Beta-D-glucoronide in rats plasma.

Key words: rats plasma, 4-dicaffeoylquinic acid, wogonin, chrysin-7-O-Beta-D-glucoronide, UHPLC-MS/MS