不同NaCl含量、培养基和微量元素对分枝杆菌SP-3降解菲的影响研究

doi:10.3976/j.issn.1002-4026.2016.06.016

Abstract

Abstract:

We addressed degradation effect of phenanthrene by Mycobacterium sp. SP3 for different salinity, culture media and trace elements. Results show that degradation rate of phenanthrene is maximum, 87.41%, when salt concentration is 1%. Degradation rate of phenanthrene is more than 80% in LB liquid media, modified LB and modified inorganic salt liquid media, no significant difference among them. Fe2+ concentration of 100 μmol/L, Zn2+ concentration of 5 μmol/L and Mg2+ concentration of 100 μmol/L can increase degradation ability of Mycobacterium sp. SP3 to phenanthrene. However, Cu2+ can inhibit degradation ability of the strain to phenanthrene with the increase of its concentration. The results will be benefit for the application of the strain to biodegradation of polycyclic aromatic hydrocarbons and the increase of its biodegradation capability to polycyclic aromatic hydrocarbons such as phenanthrene.

Key words: culture medium, Mycobacterium sp., phenanthrene, degradation rate, trace elements

CLC Number:

X172

HUANG Yu-jie, ZHANG Wen, FU Xiao-wen, GAO Yong-chao, KONG Xue, GUO Shu-hai, WANG Jia-ning. Impact of different salinity, culture media and trace elements on phenanthrene degradation of Mycobacterium sp. SP-3[J].SHANDONG SCIENCE, 2016, 29(6): 98-103.

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Impact of different salinity, culture media and trace elements on phenanthrene degradation of Mycobacterium sp. SP-3

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