生物酶对五氯联苯降解的初步研究

doi:10.3976/j.issn.1002-4026.2016.06.015

Abstract

Abstract:

Polychlorinated biphenyls (PCBs) is a representative of persistent organic pollutants (POPs). More its chlorinated number leads to more difficult its degradation . We prepared PCBs compound degrading enzyme of key enzyme formate dehydrogenase (FDH) of NADH coenzyme regeneration system with isolated 6 PCBs degradation bacteria strains as materials. We address its degrading effect to PCBs. Results indicate that degradation rate of Pseudomonas sp. MGB11/FDH compound enzyme to pentachlorodiphenyl PCB114 is up to 69.4% within 10 h. It therefore has application value in the repair for high chlorine PCBs pollutants with high toxicity and low degradability.

Key words: pentachlorodiphenyl, formate dehydrogenase, compound degrading enzyme

CLC Number:

JI Lei, CHEN Guan-hong, FU Xiao-wen, HUAGN Yu-jie, WANG Jia-ning, ZHANG Qiang. Pilot study on bioenzyme degradation to pentachlorodiphenyl[J].SHANDONG SCIENCE, 2016, 29(6): 94-97.

References

［1］董玉瑛, 冯霄. 持久性有机污染物分析和处理技术研究进展［J］. 环境污染治理技术与设备, 2003, 4（6）: 49-55.

［2］SAFE S, BANDIERA S, SAWYER T, et al. PCBs: structurefunction relationships and mechanism of action ［J］. Environmental Health Perspectives, 1985, 60: 47-56.

［3］周际海, 黄荣霞, 樊后保, 等. 污染土壤修复技术研究进展［J］. 水土保持研究, 2016, 23(3): 366-372.

［4］ABRAMOWICZ D A. Aerobic and anaerobic biodegradation of PCBs: A review ［J］. Critical Reviews in Biotechnology, 1990, 10(3): 241-251.

［5］韦朝海, 张小璇, 任源, 等. 持久性有机污染物的水污染控制: 吸附富集、生物降解与过程分析［J］. 环境化学, 2011, 30(1): 300-309.

［6］刘云兴, 王国梁. 多氯联苯生物降解技术研究进展［J］. 环境科学与管理, 2012, 37(12): 72-75.

［7］曲媛媛, 许炳雯, 丛培, 等. 两相体系中固定化联苯双加氧酶全细胞产靛蓝特性研究［J］. 大连理工大学学报, 2013, 53(3): 340-345.

［8］BRENNER V, ARENSDORF J J, FOCHT D D. Genetic construction of PCB degraders ［J］. Biodegradation, 1994, 5(3/4): 359-377.

［9］黄志华, 刘铭, 王宝光, 等. 甲酸脱氢酶用于辅酶NADH 再生的研究进展［J］. 过程工程学报, 2006, 6(6): 1011-1016.

［10］RUIZAGUILAR G M L, FERNNDEZSNCHEZ J M, RODRGUEZVZQUEZ R,et al. Degradation by whiterot fungi of high concentrations of PCB extracted from a contaminated soil ［J］. Advances in Environmental Research, 2002, 6(4): 559-568.

［11］胡星, 杜丽婷, 王斐, 等. 一株联苯基质筛选菌的生理及多氯联苯降解特性［J］. 上海大学学报 (自然科学版), 2016, 22(2): 188-196.

［12］SIERRA I, VALERA J L, MARINA M L, et al. Study of the biodegradation process of polychlorinated biphenyls in liquid medium and soil by a new isolated aerobic bacterium (Janibacter sp.) ［J］. Chemosphere, 2003, 53(6): 609-618.

［13］SAFE S. Toxicology, structurefunction relationship, and human and environmental health impacts of polychlorinated biphenyls: progress and problems ［J］. Environmental health perspectives, 1993, 100(4): 259-268.

［14］PIEPER D H, SEEGER M. Bacterial metabolism of polychlorinated biphenyls ［J］. Journal of molecular microbiology and biotechnology, 2008, 15(2/3): 121-138.

［15］FURUKAWA K, FUJIHARA H. Microbial degradation of polychlorinated biphenyls: Biochemical and molecular features ［J］ . Journal of Bioscience and Bioengineering, 2008, 105（5）: 433-449.

Metrics

Comments

Recommended 0

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0), which permits third parties to freely share (i.e., copy and redistribute the material in any medium or format) and adapt (i.e., remix, transform, or build upon the material) the articles published in this journal, provided that appropriate credit is given, a link to the license is provided, and any changes made are indicated. The material may not be used for commercial purposes. For details of the CC BY-NC 4.0 license, please visit: https://creativecommons.org/licenses/by-nc/4.0

[1]	ZHOU Chang, LIANG Heng, HUANG Lilong, ZHANG Xiaofei, WANG Jianing, SONG Fanyong, FU Xiaowen. Biotechnologies used for the remediation of oil-field soils [J]. Shandong Science, 2025, 38(2): 41-52.
[2]	WANG Yi-lian, HUANG Ding-li, WEI Yan-li, LI Hong-mei, YANG Hong-tong, LI Ji-shun. Soil conditioner and Trichoderma LTR-2 combined application for remediation of continuous cropping obstacle soil in Brassica chinensis L. [J]. Shandong Science, 2022, 35(6): 74-79.
[3]	YANG Qian,YANG Bao-shan,QIN Guang-hua,WANG Hui,SHI Jia-xing,LIU Xue-ting. Salt tolerance evaluation and selection of salt tolerance indexes for new varieties of willow seedlings [J]. Shandong Science, 2022, 35(3): 107-114.
[4]	CAO Xin-lei, JIANG Hao, YANG Bao-shan, JIAO Ke-qin, WANG Hui. Effect of silver nanoparticles on soil enzyme activity and functional diversity of microbial communities in wheat-straw-returned soil [J]. Shandong Science, 2021, 34(3): 80-89.
[5]	ZHANG Teng-fei, HUANG Yu-jie, JI Lei, WANG Jia-ning. Proceedings of bioremediation technology for petroleum contaminated soil [J]. Shandong Science, 2020, 33(5): 106-112.
[6]	HUANG Yujie, QIU Weizhong, GAO Yongchao, KONG Xue, ZHANG Wen, WANG Jianing. Isolation, identification and optimization of growth conditions of a strain of phenanthrene degrading bacteria [J]. SHANDONG SCIENCE, 2015, 28(2): 75-80.
[7]	ZHANG Qiang,ZHENG Liwen, KONG Xue, CHEN Guanhong,ZHANG Jingyu,WANG Jianing. Enhanced bioremediation of additive on petroleum contaminated soil [J]. SHANDONG SCIENCE, 2015, 28(1): 78-81.

Pilot study on bioenzyme degradation to pentachlorodiphenyl

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 7

Metrics

Comments

Recommended 0