新品种柳树幼苗耐盐性评价与耐盐指标筛选

doi:10.3976/j.issn.1002-4026.2022.03.013

Abstract

Abstract:

To explore the salt tolerance ability of different varieties of willow species, three new varieties of willow species were used to study the change characteristics of their growth and related physiological indicators under different concentrations of NaCl(20, 40, 60, and 80 mmol/L).We measured 15 parameters associated with growth physical and chemical characteristics, and the salt tolerance of three varieties of willow seedlings was evaluated comprehensively by four objective analysis methods: equal weight grey correlation analysis, entropy weight grey correlation analysis, principal component analysis and principal component-grey correlation analysis. The results showed that combining with cluster analysis the contents of soluble protein, free proline, soluble sugar, H₂O₂ and relative water content of leaves could be considered as the main indexes for the identification of salt tolerance of willow seedlings. According to four analysis methods, Salix matsudana 'Wuxu1' (cultivated code A34), Salix matsudana 'Shidi1' (cultivated code A42) present the better performance in resisting salt stress. It can be used as alternative germplasm material for exploitation and utilization of saline soil. As a scientific and reasonable evaluation method, principal component-grey correlation analysis can be one more useful appraisement method to provide theoretical support for screening and breeding of salt tolerance of willow seedlings.

Key words: :willow, salt tolerance, principal component analysis, grey correlation analysis, principal component-grey correlation analysis

CLC Number:

YANG Qian,YANG Bao-shan,QIN Guang-hua,WANG Hui,SHI Jia-xing,LIU Xue-ting. Salt tolerance evaluation and selection of salt tolerance indexes for new varieties of willow seedlings[J].Shandong Science, 2022, 35(3): 107-114.

Figures/Tables 4

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References 20

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品种	A34	A42	A7	A34-20	A42-20	A7-20	A34-40	A42-40	A7-40	A34-60	A42-60	A7-60	A34-80	A42-80	A7-80
等权关联度	0.528	0.529	0.489	0.564	0.636	0.528	0.618	0.558	0.548	0.562	0.562	0.568	0.531	0.555	0.476
排名	12	11	14	4	1	12	2	7	9	5	5	3	10	8	15
加权关联度	0.507	0.504	0.473	0.534	0.572	0.503	0.605	0.542	0.537	0.556	0.569	0.586	0.521	0.538	0.45
排名	11	12	14	9	3	13	1	6	8	5	4	2	10	7	15

指标	主成分1	主成分2	主成分3	主成分4	主成分5
游离脯氨酸	0.782	-0.050	0.214	0.463	-0.048
POD活性	-0.713	-0.164	0.628	-0.109	0.196
根冠比	0.684	0.443	0.382	0.298	0.139
可溶性蛋白含量	-0.649	0.570	-0.253	-0.175	0.241
相对电导率	0.284	0.872	0.004	0.284	-0.022
净光合速率	0.489	0.711	-0.202	0.143	0.102
CAT活性	-0.623	0.705	-0.158	0.055	-0.056
APX活性	-0.589	0.674	-0.205	0.033	-0.123
生物量变化	0.505	0.618	0.185	-0.404	-0.333
MDA含量	0.039	-0.208	0.770	0.072	0.383
可溶性糖含量	-0.333	-0.493	-0.702	0.298	0.153
类胡萝卜素含量	0.465	-0.215	-0.543	0.165	0.520
叶绿素a+b含量	-0.418	-0.308	0.507	0.402	-0.475
相对含水率	0.602	-0.168	0.050	-0.759	0.088
H₂O₂含量	0.373	-0.444	-0.509	-0.013	-0.540
初始特征值	4.323	3.801	2.692	1.472	1.223
贡献率/%	28.818	25.343	17.945	9.813	8.155
累计贡献率/%	28.818	54.161	72.106	81.919	90.074

Salt tolerance evaluation and selection of salt tolerance indexes for new varieties of willow seedlings

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