不同NaCl含量、培养基和微量元素对分枝杆菌SP-3降解菲的影响研究

doi:10.3976/j.issn.1002-4026.2016.06.016

SHANDONG SCIENCE ›› 2016, Vol. 29 ›› Issue (6): 98-103.doi: 10.3976/j.issn.1002-4026.2016.06.016

• Environment and Ecology • Previous Articles Next Articles

Impact of different salinity, culture media and trace elements on phenanthrene degradation of Mycobacterium sp. SP-3

HUANG Yu-jie¹, ZHANG Wen¹, FU Xiao-wen¹, GAO Yong-chao¹, KONG Xue¹, GUO Shu-hai^{1, 2}, WANG Jia-ning^1*

1. Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Shandong Academy of Sciences, Jinan 250014, China;2. Applied Ecology Institute, Chinese Academy of Sciences, Shenyang 110016, China

Received:2016-03-22 Online:2016-12-20 Published:2016-12-20

Abstract

Abstract:

We addressed degradation effect of phenanthrene by Mycobacterium sp. SP3 for different salinity, culture media and trace elements. Results show that degradation rate of phenanthrene is maximum, 87.41%, when salt concentration is 1%. Degradation rate of phenanthrene is more than 80% in LB liquid media, modified LB and modified inorganic salt liquid media, no significant difference among them. Fe2+ concentration of 100 μmol/L, Zn2+ concentration of 5 μmol/L and Mg2+ concentration of 100 μmol/L can increase degradation ability of Mycobacterium sp. SP3 to phenanthrene. However, Cu2+ can inhibit degradation ability of the strain to phenanthrene with the increase of its concentration. The results will be benefit for the application of the strain to biodegradation of polycyclic aromatic hydrocarbons and the increase of its biodegradation capability to polycyclic aromatic hydrocarbons such as phenanthrene.

Key words: culture medium, Mycobacterium sp., phenanthrene, degradation rate, trace elements

CLC Number:

X172

HUANG Yu-jie, ZHANG Wen, FU Xiao-wen, GAO Yong-chao, KONG Xue, GUO Shu-hai, WANG Jia-ning. Impact of different salinity, culture media and trace elements on phenanthrene degradation of Mycobacterium sp. SP-3[J].SHANDONG SCIENCE, 2016, 29(6): 98-103.

References

［1］姜岩, 杨颖, 张贤明. 典型多环芳烃生物降解及转化机制的研究进展［J］. 石油学报(石油加工), 2014. 30(6): 1137-1150.

［2］GOLDMAN R. Smoking increase carcinogenic polycyclic aromatic hydrocarbons in human lung tissue［J］. Cancer Res, 2001. 61：6347-6371.

［3］MOUTTAKI H, JOHANNES J, MECKENSTOCK U R. Identification of naphthalene carboxylase as a prototype for the anaerobic activation of non-substituted aromatic hydrocarbons［J］. Environmental Microbiolgy, 2012. 14(10): 2770-2774.

［4］陈春云, 岳珂, 陈振明, 等. 微生物降解多环芳烃的研究进展［J］. 微生物学杂志, 2007. 27(6): 100-103.

［5］马静, 曹亚明, 徐丽, 等. Pseudomonas sp. JM2菲降解酶基因解析［J］. 环境科学与技术, 2013. 36(12): 15-19.

［6］YANG Y, ZHANG N, XUE M, et al. Effects of organic matter on the development of the microbial polycyclic aromatic hydrocarbons (PAHs) degradation potentials［J］. Environmental Pollution, 2011, 159(2): 591-595.

［7］陈芳艳, 粱林林, 唐玉斌, 等. 一株菲降解菌的分离鉴定及固定化条件研究［J］. 环境科学与技术, 2007. 30(12): 19-21.

［8］袁爽, 梁成华, 李凤梅, 等．多环芳烃降解菌的筛选与降解能力测定［J］.生态学杂志, 2011, 30( 2): 315-319.

［9］ZHAO H P, WU Q S, WANG L, et al．Degradation of phenanthrene by bacterial strain isolated from soil in oil refinery fields in Shanghai, China［J］．Journal of Hazardous Materials, 2009. 164( 23): 863-869.

［10］STORY S P, PARKER S H, KLINE J D, et al. Identification of four structural genes and two putative promoters necessary for utilization of naphthalene, phenanthrene, and fluoranthene by Sphingomonas paucimobilis var. EPA505［J］. Gene, 2000, 260(1/2): 155-169.

［11］邢维芹, 骆永明, 李立平. 影响土壤中PAHs 降解的环境因素及促进降解的措施［J］.土壤通报, 2007, 38( 1): 173-178.

［12］WONG J W, LAI K M, WAN C K, et al. Isolation and optimization of PAHdegradative bacteria from contaminated soil for PAHs bioremediation［J］. Water, Air, & Soil Pollution, 2002, 139(1): 1-13.

［13］思显佩, 曹霞霞, 熊建功. 微生物降解多环芳烃的影响因素及机理研究进展［J］. 重庆工商大学学报( 自然科学版), 2009, 26( 5): 457-461.

［14］黄玉杰, 邱维忠, 高永超, 等. 一株菲降解菌的分离、鉴定及最佳培养条件的优化［J］. 山东科学, 2015, 28(2): 75-80.

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Impact of different salinity, culture media and trace elements on phenanthrene degradation of Mycobacterium sp. SP-3

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