SHANDONG SCIENCE ›› 2016, Vol. 29 ›› Issue (5): 90-97.doi: 10.3976/j.issn.1002-4026.2016.05.015

• Biosensors • Previous Articles     Next Articles

Development of biosensors based online analysis system for fermentation process

ZHU Sirong, ZHOU Wanli, BI Chunyuan, ZHANG Liqun, YANG Yan, ZHANG Jinling,ZHAO Xiaohua   

  1. Shandong Provincial Key Laboratory of Biosensors, Institute of Biology, Shandong Academy of Sciences, Jinan 250014,China
  • Received:2016-07-29 Published:2016-10-20 Online:2016-10-20

Abstract:

We developed a biosensors based online automatic sampling and analysis system in fermentation process for the requirements of automatic optimization control in fermentation process. We presented its analytical principle and structure components and detailed its automatic sampling, sample dilution and biosensor signal processing. It can automatically analyze such important biochemical parameters as glucose, lactic acid and glutamic acid, and send analytical results to fermentation controller through a 485 interface or a 4~20 mA analog interface. It can therefore provide biochemical analytical data for fermentation control. We further tested experiment prototype with glucose standard sample as fermentation broth. Results show that it has higher analytical accuracy and can satisfy the requirements of fermentation process analysis.

Key words: biosensor, automatic analyzer, glucose analysis, lactic acid analysis, automatic sampling from fermentation tank, online analysis

CLC Number: 

  • Q815

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