霜霉病高效生防菌株BCJB01发酵培养基的优化

doi:10.3976/j.issn.1002-4026.2016.04.007

Abstract

Abstract:

To improve fermentation level of Bacillus cereus BCJB01, a highly efficient biocontrol strain against downy mildew, we optimized its fermentative medium through orthogonal test, comprehensive optimization and sugar feeding tests. Mass fraction of the optimized medium formula are 2.00% corn meal,1.00% soybean powder, 0.0030% manganese chloride tetrahydrate, 0.30% twelve aqueous disodium hydrogen phosphate, 0.15% sodium dihydrogen phosphate dihydrate, 0.035% magnesium sulfate heptahydrate, 0.10% calcium chloride, 0.30% yeast extract, 0.2%sugar added at 6~12 h after inoculation. It can produce more than 100×108 cfu/mL cells and its spore rate is 90%, when the cultivation conditions are 3%(V/V) inoculum size, 180 r / min rotation velocity, 30 ℃ shaky cultivation till 66 hours.

Key words: orthogonal design, downy mildew, Bacillus cereus, fermentative medium, comprehensive optimization

CLC Number:

Q939.95

WANG Yi-lian,XIE Xue-ying,HU Jin-dong,CHEN Kai,YANG He-Tong,LI Ji-shun. Optimization of fermentative medium for highly effective biocontrol strain Bacillus cereus BCJB01 against downy mildew[J].SHANDONG SCIENCE, 2016, 29(4): 30-34.

References

［1］杨柳燕,徐永阳,徐志红,等. 甜瓜霜霉病研究进展［J］.中国瓜菜, 2011, 24(3): 38-43.
［2］李宝燕,王英姿,刘学卿,等.3 种杀菌剂对葡萄霜霉病菌的毒力测定和田间药效试验［J］.江苏农业科学,2014,42(1):98-99.
［3］李宝燕,王培松,王英姿.葡萄霜霉病的生物药剂防治［J］.农药,2014,53(11): 853-855.
［4］秦文韬. 葡萄霜霉病菌快速分子检测方法研究［D］.北京：中国农业科学院.2013.
［5］刘婧,马汇泉,董瑾,等.蜡样芽孢杆菌B02菌株培养基及培养条件的优化［J］.山东农业科学,2008(7):54-57.
［6］PERAZZOLLIM, MORETTOM, FONTANA P, et al. Downy mildew resistance induced by Trichoderma harzianum T39 in susceptible grapevines partially mimics transcriptional changes of resistant genotypes ［J］. BMC Genomics 2012, 13:660.
［7］岳宪化,胡夫防,段丽峰,等.哈茨木霉菌防治葡萄霜霉病试验［J］.中国果树,2014(2):54-56.
［8］陈浩,胡梁斌,唐春平,等.枯草芽胞杆菌BFS01对葡萄霜霉病的防治效果［J］.植物保护,2011,37(6):194-197.
［9］郭继平,马光,朱会霞,等. 葡萄霜霉病拮抗细菌N6的分离、筛选及鉴定［J］.湖北农业科学,2014,53(17):4063-4065.
［10］扈进冬,吴远征,李纪顺,等.拮抗性多粘类芽孢杆菌PB2及其制剂对葡萄霜霉病的防效测定［J］.山东科学,2014,27(3):30-33.
［11］臧超群,赵奎华,刘长远,等.生防菌株SY286 对葡萄霜霉病菌的抑菌效果［J］.沈阳农业大学学报,2014,45(2): 221-224.
［12］朱玥妍,刘姣,杜春梅.芽孢杆菌生物防治植物病害研究进展［J］.安徽农业科学,2012,40(34):16635-16638.
［13］唐容容,杨文革,胡永红,等.蜡样芽孢杆菌CGMCC4348菌株防治番茄灰霉病的效果及机理研究［J］.湖北农业科学,2013,52(8):1817-1820.
［14］朱天辉,李姝江,韩珊,等.杂交竹白纹羽病生防菌的筛选与应用［J］.四川林业科技,2013,34(6):8-12.
［15］彭双,闫淑珍,陈双林.具杀线虫活性植物内生细菌的筛选和活性产物［J］.微生物学报,2011,51(3):368-376.
［16］王刚, 刘凤英,王淼,等.内生细菌B37的运动性参与其在小麦根系的内生定殖和对小麦全蚀病的生物防治［J］.植物病理学报, 2011, 41(5):526-533.
［17］黄秋斌,张颖,刘凤英,等.蜡样芽孢杆菌B37在大田小麦根部的定殖动态及其对小麦纹枯病的防治效果［J］.生态学报,2014,34(10): 2559-2566.
［18］徐迟默,谢龙莲,曾筱芬,等.荔枝霜霉病生物防治研究进展［J］.南方农业学报,2015,46(1):72-78.
［19］张文芝,王云鹏,刘红霞,等.蜡质芽孢杆菌AR156 发酵培养基及发酵条件的优化［J］.微生物学通报,2010,37(6):803-810.
［20］梁昌聪,郭立佳,刘磊,等.响应面法优化解淀粉芽孢杆菌 C101 发酵培养基［J］.生物技术通报,2014 (8) :169-174.

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Optimization of fermentative medium for highly effective biocontrol strain Bacillus cereus BCJB01 against downy mildew

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