SHANDONG SCIENCE ›› 2016, Vol. 29 ›› Issue (4): 30-34.doi: 10.3976/j.issn.1002-4026.2016.04.007

• Agricultural Microbiology • Previous Articles     Next Articles

Optimization of fermentative medium for highly effective biocontrol strain Bacillus cereus BCJB01 against downy mildew

WANG Yi-lian,XIE Xue-ying,HU Jin-dong,CHEN Kai,YANG He-Tong,LI Ji-shun*   

  1. Shandong Provincial Key Laboratory of Applied Microbiology, Biology Institute, Shandong Academy of Sciences, Jinan 250014, China
  • Received:2016-04-27 Online:2016-08-20 Published:2016-08-20

Abstract:

To improve fermentation level of Bacillus cereus BCJB01, a highly efficient biocontrol strain against downy mildew, we optimized its fermentative medium through orthogonal test, comprehensive optimization and sugar feeding tests. Mass fraction of the optimized medium formula are 2.00% corn meal,1.00% soybean powder, 0.0030% manganese chloride tetrahydrate, 0.30% twelve aqueous disodium hydrogen phosphate, 0.15% sodium dihydrogen phosphate dihydrate, 0.035% magnesium sulfate heptahydrate, 0.10% calcium chloride, 0.30% yeast extract, 0.2%sugar added at 6~12 h after inoculation. It can produce more than 100×108 cfu/mL cells and its spore rate is 90%, when the cultivation conditions are 3%(V/V) inoculum size, 180 r / min rotation velocity, 30 ℃ shaky cultivation till 66 hours.

Key words: orthogonal design, downy mildew, Bacillus cereus, fermentative medium, comprehensive optimization

CLC Number: 

  • Q939.95