镰刀菌（Fusarium sp.）ZW-21的鉴定及其利用玉米秸秆水解液发酵产乙醇研究

doi:10.3976/j.issn.1002-4026.2015.06.011

Abstract

Abstract: We identified a filamentous fungus strain Fusarium sp. ZW21 that could ferment corn straw hydrolysate and produced ethanol. It was identified as Fusarium genus through 18S rDNA sequence analysis.We discovered it could produce ethanol with corn straw hydrolysate.We further investigated the impact of sugar concentration of corn straw hydrolysate, nitrogen sources, pH value, temperature, inoculum volume and surface active agents on ethanol production.Two factors of yeast extract and Tween 80 were selected to further optimize these influential factors by response surface methodology. Optimal medium were eventually acquired: 50 g/L straw hydrolysate, 10.19 g/L yeast extract,10 g/L KH2PO4, 0.5 g/L MgSO4·7H2O,0.423 g/L Tween 80, pH value of 6.0, 8% (V/V)inoculum volume, 32 ℃ incubation temperature, and 5 days fermentation time with limited oxygen.The highest ethanol yield was 22.1 g/L.

Key words: corn straw hydrolysate, fermentation, bioethanol, optimization, Fusarium sp.

CLC Number:

X172

WANG Binglian,FENG Dong, LIANG Xiaohui, LI Dahai. Identification of strain Fusarium sp. ZW21 and bioethanol production by corn straw hydrolysate fermentation[J].SHANDONG SCIENCE, 2015, 28(6): 65-72.

References

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Identification of strain Fusarium sp. ZW21 and bioethanol production by corn straw hydrolysate fermentation

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