碳氮元素数量比对典型耐盐微生物增殖影响

doi:10.3976/j.issn.1002-4026.20240128

Abstract

Abstract:

Bacillus velezensis, Bacillus thuringiensis and Brevibacterium frigoritolerans are three typical salt-tolerant plant growth-promoting rhizobacteria (ST-PGPR) known for their ability to alleviate salt stress in plants. To optimize the nutritional conditions for the growth and proliferation of these strains, experiment of single factor method was used to investigate the effects of different carbon-to-nitrogen ratios (n_C/n_N) on their development. The results showed that all three strains could grow and reproduce in a medium with a n_C/n_N of 3.25~6.00. However, the n_C/n_N had varying effects on their proliferation, i.e., the most significant impact was observed on the proliferation of velezensis, followed by thuringiensis and frigoritolerans. When the n_C/n_N was 4:1, velezensis exhibited the highest proliferation, with a viable bacterial count of 6.2 × 10⁸ CFU/mL. Similarly, thuringiensis achieved its highest proliferation at a n_C/n_N of 4:1, with a viable bacterial count of 5.1 × 10⁸ CFU/mL. Conversely, frigoritolerans achieved its highest proliferation at a n_C/n_N of 5:1, with a viable bacterial count of 3.5 × 10⁸ CFU/mL. In conclusion, the optimal n_C/n_N for the proliferation of velezensis and thuringiensis is 4:1, whereas that for frigoritolerans is 5:1.

Key words: salt-tolerant microorganisms, carbon-to-nitrogen ratios, medium optimization, microbial biomass

CLC Number:

Q939.9

WANG Chen, LU Yaxin, LI Xiaoyong, ZHANG Jiayi, XU Letian, FENG Qing. Effects of carbon-to-nitrogen ratio on the proliferation of typical salt-tolerant microorganisms[J].Shandong Science, 2025, 38(2): 73-79.

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References 24

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实验组	m(葡萄糖)/g	m(蛋白胨)/g	碳氮比	m(KH₂PO₄)/g	m(MgSO₄·7H₂O)/g
1	0	1.0	3.25:1	0.2	0.5
2	0.2	1.0	4:1	0.2	0.5
3	1.2	1.0	7:1	0.2	0.5
4	2.1	1.0	10:1	0.2	0.5

碳氮比	生物量(OD₆₀₀)	菌种数量×10^-8/(CFU·mL^-1)
3.25:1	2.165	3.2
4:1	2.284	4.5
7:1	2.028	1.3
10:1	2.005	0.3

实验组	m(葡萄糖)/g	m(蛋白胨)/g	碳氮比	m(KH₂PO₄)/g	m(MgSO₄·7H₂O)/g
1	0	1.0	3.25:1	0.2	0.5
2	0.2	1.0	4:1	0.2	0.5
3	0.5	1.0	5:1	0.2	0.5
4	0.9	1.0	6:1	0.2	0.5

碳氮比 (n_C/n_N)	生物量(OD)			菌种数量×10^-8/(CFU·mL^-1)
碳氮比 (n_C/n_N)	苏云金芽孢杆菌	贝莱斯芽孢杆菌	耐寒短杆菌	苏云金芽孢杆菌	贝莱斯芽孢杆菌	耐寒短杆菌
3.25:1	2.235	2.322	2.117	3.4	3.6	1.7
4:1	2.292	2.523	2.187	5.1	6.2	2.4
5:1	2.183	2.307	2.236	2.6	4.7	3.5
6:1	2.030	2.273	2.191	1.5	3.8	2.6

Effects of carbon-to-nitrogen ratio on the proliferation of typical salt-tolerant microorganisms

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