豆豉姜中生物碱的pH区带逆流色谱分离研究

doi:10.3976/j.issn.1002-4026.2015.04.008

SHANDONG SCIENCE ›› 2015, Vol. 28 ›› Issue (4): 41-45.doi: 10.3976/j.issn.1002-4026.2015.04.008

• Traditional Chinese medicine and natural active products • Previous Articles Next Articles

Separation and purification of alkaloids from Litsea cubeba (Lour．)Pers. by pHzonerefining countercurrent chromatography

SUN Li1,GONG Chengyu2,YANG Peng3, CHEN Yanping2,YANG Bingtian3,LIU Jianhua4*

1.Zibo Municipal Traditional Chinese Medicine Hospital, Zibo 255300, China;2.Yantai Entryexit Inspection and Quarantine Bureau, Yantai 264000, China;3.Sanfeng Biological Engineering Technology Co.Ltd., Jinan 250014, China;4.Shandong Analysis and Test Center, Jinan 250014，China

Received:2015-07-01 Published:2015-08-20 Online:2015-08-20

Abstract

Abstract: We construct a method for separating and purifying alkaloids from Litsea cubeba(Lour．) Pers.by pHzonerefining countercurrent chromatography (CCC).Our experimental conditions are solvent of chloroformmethanolwater(4:3:2,V/V),upper stationary phase of 70 mmol/L HCl, lower mobile phase of 10 mmol/L TEA, revolution speed of 800 r/min, flow rate of 2 mL/min, detection wavelength of 254 nm, and stationary phase retention rate of 65%.We acquire 246 mg of boldine, 524 mg of laurotetanine and 317 mg of isoboldine from 1.5 g crude extract of Litsea cubeba(Lour．) Pers.through one time pHzonerefining CCC.Their purities are 97.35%, 95.48% and 97.54% by HPLC analysis.Results show that pHzonerefining CCC can effectively separate and prepare major active components of Litsea cubeba(Lour．) Pers.,aporphine alkaloids.

Key words: Litsea cubeba(Lour．) Pers., pHzonerefining countercurrent chromatography, alkaloids

CLC Number:

R284.2

SUN Li1,GONG Chengyu2,YANG Peng3, CHEN Yanping2,YANG Bingtian3,LIU Jianhua4*. Separation and purification of alkaloids from Litsea cubeba (Lour．)Pers. by pHzonerefining countercurrent chromatography[J].SHANDONG SCIENCE, 2015, 28(4): 41-45.

References

［1］张水英, 郭强, 高小力，等.樟科药用植物山鸡椒的化学成分和药理活性研究进展［J］.中国中药杂志, 2014, 39( 5): 769-776. ［2］国家药典委员会.中华人民共和国药典2010年版一部［M］.北京: 中国医药科技出版社, 2010. ［3］LIN Ｂ，ZHANG H，ZHAO X X, et al.Inhibitory effects of the root extract of Litsea cubeba(Lour.) Pers.on adjuvant arthritis in rats ［J］.J Ethnopharmacol, 2013, 147( 2): 327-334. ［4］ITO Y, MA Y. pHzonerefining countercurrent chromatography ［J］.J Chromatogr A, 1996, 753（１）:1-36. ［5］ITO Y. Golden rules and pitfalls in selecting optimum conditions for highspeed countercurrent chromatography ［J］.J Chromatogr A, 2005, 1065( 2): 145-168. ［6］ITO Y. pHzonerefining countercurrent chromatography: Origin, mechanism, procedure and applications ［J］.J Chromatogr A, 2013, 1271（1）: 71-85. ［7］PAN Y J, LU Y B.Recent progress in countercurrent chromatography ［J］.J Chromatogr Relat Technol, 2007, 30（5/6/7/8）: 649-679. ［8］YU Q, TONG S Q, YAN J Z, et al.Preparative separation of quaternary ammonium alkaloids from Corydalis yanhusuo W.T.Wang by pHzonerefining countercurrent chromatography ［J］.J Sep Sci, 2011, 34(3): 278-285. ［9］WANG X, LIU J H, GENG Y L, et al.Preparative separation of alkaloids from Nelumbo nucifera Gaertn by pHzone refining countercurrent chromatography ［J］.J Sep Sci, 2010, 33(4/5): 539-544. ［10］WEISZ A, MAZZOLA E P, ITO Y.Preparative separation of 1, 3, 6pyrenetrisulfonic acid trisodium salt from the color additive D&C Green No.8 (pyranine) by pHzonerefining countercurrent chromatography ［J］.J Chromatogr A, 2011, 1218(45): 8249-8254. ［11］MA Y, ITO Y.Peptide separation by pHzonerefining countercurrent chromatography ［J］.J Chromatogr A, 1997, 771(1/2): 81-88. ［12］SUN C L, LI J, WANG X, et al.Preparative separation of quaternary ammonium alkaloids from Coptis chinensis Franch by pHzonerefining countercurrent chromatography ［J］.J Chromatogr A, 2014, 1370: 156-161. ［13］严小红, 魏孝义, 谢海辉, 等.豺皮樟和圆叶豺皮樟中的阿朴啡生物碱成分［J］.热带亚热带植物学报, 2000, 8(4): 324-328. ［14］曲郁虹, 姜明, 王玲燕, 等.华南木姜子的阿朴菲型生物碱成分［J］.中国中药杂志, 2015, 40 (1): 94-97. ［15］张水英, 郭强, 曹愿, 等.豆豉姜的生物碱类成分研究［J］.中国中药杂志, 2014, 39 (20): 3964-3968.

Metrics

Comments

Recommended 0

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0), which permits third parties to freely share (i.e., copy and redistribute the material in any medium or format) and adapt (i.e., remix, transform, or build upon the material) the articles published in this journal, provided that appropriate credit is given, a link to the license is provided, and any changes made are indicated. The material may not be used for commercial purposes. For details of the CC BY-NC 4.0 license, please visit: https://creativecommons.org/licenses/by-nc/4.0

[1]	ZHU Heng, WEN Lei,GENG Yanling, WANG Xiao, JIA Wenting,WANG Daijie,YAN Yunhong. Antioxidant activity of alkaloids in Caulis Mahoniae [J]. SHANDONG SCIENCE, 2016, 29(5): 24-28.
[2]	ZHU Rong-Hua, WANG Xiao, LIU Jian-Hua, ZHANG Shu-Qin, LIU Feng. Extraction and enrichment of total alkaloids of Plumula Nelumbinis by ultrahigh pressureassisted micellar extraction combined with cloudpoint extraction [J]. J4, 2013, 26(3): 15-20.

Separation and purification of alkaloids from Litsea cubeba (Lour．)Pers. by pHzonerefining countercurrent chromatography

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 2

Metrics

Comments

Recommended 0