Abstract:

We establish a HPLC based method for determining the contents of rutin, hyperoside and quercetin from Bidens parviflora Willd. The linear ranges of rutin, hyperoside and quercetin are 0.50~12.50 μg/mL(r=0.999 9), 0.49~12.35 μg/mL(r=0.999 8), 0.42~10.55 μg/mL (r=0.999 5), and the average recovery rates (n=3) are 99.37%(RSD=0.56%), 99.35%(RSD=0.57%), 99.68%(RSD=0.56) under the conditions of DiamonsilC18column, mobile phase of acetonitrile0.2% glacial acetic acid, flow rate of 1 mL/min and the detection wavelength of 360 nm. The method is simple, accurate and repeatable and can be employed to determine three flavonoid contents from Bidens parviflora Willd.

Key words: Bidens parviflora Willd., rutin, hyperoside, quercetin, HPLC