Abstract: To explore the potential mechanism of anti-myocardial ischemia of Pseudostellariae Radix, the active components and targets associated with myocardial ischemia were predicted using network pharmacology, and the key target network of anti-myocardial ischemia was constructed. GO（gene ontology) function and Pathway enrichment analysis were conducted, and molecular docking verification was performed between the active components and the key targets. Eight active components of Pseudostellariae Radix and 50 key targets of anti-myocardial ischemia were obtained. GO function and Pathway enrichment analysis showed that anti-myocardial ischemia effects of Pseudostellariae Radix were mainly involved in biological processes such as apoptosis, angiogenesis, and inflammation, including MyD88-independent TLR4 cascade (MyD88-independent TLR4 signal transduction pathway), MyD88:MAL cascade initiated on plasma membrane, and TLR4 signaling (activated TLR4 signal pathway). The main targets included JUN, MAPK1, IKBKG, PTPN11, and RELA. Molecular docking showed that dandelion terpenol had the best binding with the key targets AKT1, IL6, MAPK1 and TP53. The mechanism of anti-myocardial ischemia of Pseudostellariae Radix through multiple pathway and multiple targets was revealed in this study, which provides a basis for further study of the anti-myocardial ischemia effect of Pseudostellariae Radix.

Key words: Pseudostellariae Radix, myocardial ischemia, network pharmacology, molecular docking