Shandong Science ›› 2019, Vol. 32 ›› Issue (3): 80-84.doi: 10.3976/j.issn.1002-4026.2019.03.012

• Environment and Ecology • Previous Articles     Next Articles

Determination of diethylstillbestrol residues in aquatic products by enzyme linked immunosorbent assay

ZHANG Rui-ling1, XU Yi-zhen1, LIU Jian-ping2, LI Fu-wei1, ZHANG Chong1   

  1. 1.Shandong Analysis and Test Center, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250014, China; 2.Shandong Bluetown Analysis and Teat Co., LTD, Jinan 250014, China
  • Received:2018-06-08 Online:2019-06-20 Published:2019-06-01

Abstract: The content of diethylstilbestrol(DES)residues in aquatic products was determined by enzyme linked immunosorbent assay(ELISA),and the positive results were verified by liquid chromatography-mass spectrometry (LC-MS).The final test results show that the detection limit of DES in aquatic products is 0.35 μg/kg and the recovery rate is 77%~91%. This method has good sensitivity and accuracy.

Key words: Enzyme linked immunosorbent assay (ELISA), diethylstilbestrol (DES), aquatic products

CLC Number: 

  • O657.63