木霉属中国新记录种Trichoderma paratroviride

doi:10.3976/j.issn.1002-4026.2015.05.006

Abstract

Abstract: Trichoderma strain Y15 was isolated from the vegetable soil under Shandong greenhouse, and identified by analysis of internal transcribed spacer regions of the rRNA gene cluster (ITS), partial sequences of transcription extensions factor 1alpha (tef1α) and morphological characteristics. Results show that Y15 is T. paratroviride. The strain is colony dense, panniform, greyishgreen on PDA. Conidiophore main axes is long with short secondary branches, not extensively rebranching. Its branches are coupled or verticillate. Phialides usually has 2~4 verticils, lageniform or subulate, straight or flexuous. Conidia is subglobose to obovoid, greenish and smooth. The tef1α sequence of strain Y15 has 99% homology with strains S489 and S385 of T. paratroviride type, and has the closest genetic relationship with them in phylogenetic tree. It is originally reported in China as a new Chinese record of genus Trichoderma.

Key words: Trichoderma paratroviride, ITS, tef1-α, Chinese new record

CLC Number:

Q949.32

ZHANG Guangzhi, ZHANG Xinjian,CHEN Kai,WU Xiaoqing,LI Jishun,YANG Hetong. Trichoderma paratroviride,Chinese new record of Trichoderma[J].SHANDONG SCIENCE, 2015, 28(5): 35-40.

References

［1］HARMAN G E, HOWELL C R, VITERBO A, et alTrichoderma speciesopportunistic, avirulent plant symbionts［J］Nature Reviews Microbiology, 2004, 2: 43-56 ［2］CONTRERASCORNEJO H A, MACIASRODRIGUEZ L, CORTESPENAGOS C, et alTrichoderma virens, a plant beneficialfungus, enhances biomass production and promotes lateral root growth through an auxindependent mechanism in Arabidopsis［J］ Plant Physiology, 2009, 149: 1579-1592 ［3］LORITO M, WOO S L, HARMAN G E, et al Translational research on Trichoderma:From ‘Omics to the field［J］ Annual Review of Phytopathology, 2010, 48: 395-417 ［4］AHAMED A, VERMETTE P Culturebased strategies to enhance cellulase enzyme production from Trichoderma reesei RUTC30 in bioreactor culture conditions［J］ Biochemical Engineering Journal, 2008, 40(3): 399-407 ［5］Van NGUYEN N, KIM Y J, OH K T, et al Antifungal activity of chitinases from Trichoderma aureoviride DY59 and Rhizopus microsporus［J］ Current Microbiology, 2008, 56: 2832 ［6］SINGH H B, SINGH D P From biological control to bioactive metabolites: prospects with Trichoderma for safe human food［J］ Pertanika Journal of Tropical Agriculture Sciences, 2009, 32(1): 99-110 ［7］KREDICS L, ANTAL L, MANCZINGER L, et al Breeding of mycoparasitic Trichoderma strains for heavy metal resistance［J］ Letters in Applied Microbiology, 2001, 33(2): 112-116 ［8］HARMAN G E, LORITO M, LYNCH J M Uses of Trichoderma spp to remediate soil and water pollution［J］ Advances In Applied Microbiology, 2004, 56: 313-330 ［9］EZZI M I, LYNCH J M Biodegradation of cyanide by Trichoderma spp and Fusarium spp［J］ Enzyme and Microbial Technology, 2005, 36(7): 849-854 ［10］田晔，滕应 木霉属真菌在重金属污染土壤生物修复中的应用潜力分析［J］ 科学技术与工程，2013, 36(13): 10877-10882 ［11］HARISH R, SUPREETH M , CHAUHAN J B Biodegradation of organophosphate pesticide by soil fungi［J］ Advanced BioTech, 2013, 12(9): 4-8 ［12］TRIPATHI P, SINGH P C, MISHRA A, et al Trichoderma: a potential bioremediator for environmental clean up［J］ Clean Technologies and Environmental Policy, 2013, 15（4）: 541-550. ［13］CHAVERRI P, BRANCOROCHA F, JAKLITSCH W, et al Systematics of the Trichoderma harzianum species complex and the reidentification of commercial biocontrol strains［J］ Mycologia, 2015, 107(3):558-590 ［14］JAKLITSCH W M, VOGLMAYR H Biodiversity of Trichoderma (Hypocreaceae) in Southern Europe and Macaronesia［J］ Studies in Mycology, 2015, 80: 1–87 ［15］张广志，杨合同，张新建，等 木霉现有种类名录［J］ 菌物学报，2014, 33(6): 1210-1230 ［16］ZHU Z X, ZHUANG W Y Three new species of Trichoderma with hyaline ascospores from China［J］ Mycologia, 2015, 107(2）：328-345 ［17］朱兆香，庄文颖 中国木霉属两个新种［J］ 菌物学报，2014, 33 (6): 1168-1174 ［18］朱兆香，庄文颖 木霉属12个中国新记录种和3个新组合［J］ 菌物学报, 2014, 33(6):1175-1209 ［19］孙军，段玉玺，吕国忠 辽宁木霉属（Trichoderma）真菌的形态分类研究［J］ 菌物研究, 2006, 4(2): 38-44 ［20］ZHANG C L, XU TTrichoderma species from China［J］ Journal of Zhejiang University :Agriculture & Life Sciences, 2004, 30(4): 464 ［21］ZHANG C L, LIU S P, LIN F C, et alTrichoderma taxi spnov, an endophytic fungus from Chinese yew Taxus mairei［J］ Federation of European Microbiological Societies Microbiology Letters, 2007, 270(1): 90-96 ［22］YU Z F, QIAO M, ZHANG Y, et al Two new species of Trichoderma from Yunnan, China［J］ Antonie van Leeuwenhoek, 2007, 92(1): 101-108 ［23］YUAN Z L, CHEN Y C, ZHANG C L, et alTrichoderma chlorosporum,a new record of endophytic fungi from Dendrobium nobile in China［J］ 菌物学报, 2008, 27(4): 608-610 ［24］贾东晨，余泽芬，乔敏，等 木霉属中国新记录种［J］ 菌物学报，2009, 28(6): 860-862 ［25］顾金刚，李世贵，刘卫星，等 木霉属真菌的分离与鉴定［C］//中国微生物学会微生物资源专业委员会成立大会暨第一届学术研讨会论文集北京, 2009 ［26］李广记，陈捷，刘铜，等 木霉属中国新记录种Trichoderma koningiopsis记述［J］ 微生物学通报，2010, 37(11): 1663-1665 ［27］SUN R Y, LIU Z C, FU K H, et alTrichoderma biodiversity in China［J］ Journal of Applied Genetics, 2012, 53(3): 343-354 ［28］张广志，杨合同，张新建，等 木霉属中国新纪录种Trichoderma pleuroticola和T pleurotum［J］微生物学通报，2013, 40(4): 626-630 ［29］LI Q R, TAN P, JIANG Y L, et al A novel Trichoderma species isolated from soil in Guizhou,T guizhouense［J］ Mycological Progress, 2013, 12（2）: 167-172 ［30］崔岩，王蒂，柴兆祥，等 中国木霉新记录种俄罗斯木霉的分离鉴定及对马铃薯干腐病菌和黑痣病菌的拮抗作用［J］ 草叶学报，2014, 23（1）：276-282 ［31］CHAVERRI P, SAMUELS G JHypocrea/Trichoderma: Ascomycota, Hypocreales, Hypocreaceae: species with green ascospores［J］ Study in Mycology, 2003, 48:1-116 ［32］LU B S, DRUZHININA I S, FALLAH P, et alHypocrea/Trichoderma species with pachybasium-like conidiophores: teleomorphs for Tminutisporum and T polysporum and their newly discovered relatives［J］ Mycologia, 2004, 96(2):310-342 ［33］JAKLITSCH W M, SAMUELS G J, DODD S L, et alHypocrea rufa/Trichoderma viride: a reassessment, and description of five closely related species with and without warted conidia［J］Studies in Mycology, 2006, 55:135-177 ［34］JAKLITSCH W M, SAMUELS G J, ISMAIEL A, et al  Disentangling the Trichoderma viridescens complex［J］ Persoonia, 2013, 31(8):112-146 ［35］WHITE T J, BRUNS T, LEE S, et al Amplification and directsequencing of fungal ribosomal RNA genes for phylogenetics［C］// PCR protocols: a guide to methods and applications New York: Academic Press,Inc, 1990: 315-322 ［36］CARBONE I, KOHN L M A method for designing primer sets for speciation studies in filamentous ascomycetes［J］ Mycologia, 1999, 91(3): 553-556 ［37］JAKLITSCH W M, KOMON M, KUBICEK C P, et alHypocrea voglmayrii sp nov from the Austrian Alps represents a new phylogenetic clade in Hypocrea/ Trichoderma［J］ Mycologia, 2005, 97(6): 1365- 1378 ［38］YABUKI T, MIYAZAKI K, OKUDA T Japanese species of the longibrachiatum clade or Trichoderma［J］ Mycoscience, 2014, 55(3): 196-212 ［39］CHAVERRI P, GAZIS R O, SAMUELS G JTrichoderma amazonicum,a new endophytic species on Hevea brasiliensis and H guianensis from the Amazon basin［J］ Mycologia, 2011, 103(1): 139-151

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Trichoderma paratroviride,Chinese new record of Trichoderma

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