山东科学 ›› 2018, Vol. 31 ›› Issue (4): 31-38.doi: 10.3976/j.issn.1002-4026.2018.04.006

• 中药与天然活性产物 • 上一篇    下一篇

不同株系丹参有效成分比较研究

梁从莲,张永清,李佳,刘谦,蒲高斌*   

  1. 山东中医药大学,山东 济南 250355
  • 收稿日期:2017-11-10 出版日期:2018-08-20 发布日期:2018-08-20
  • 通信作者: 蒲高斌,副教授,研究方向为中药资源及其质量控制。E-mail:gbpu@163.com E-mail:gbpu@163.com
  • 作者简介:梁从莲(1990—),女,博士研究生,研究方向为中药资源及其质量控制。
  • 基金资助:

    山东省高等学校科技计划 (J15LM55);山东省重点研发计划(2016GSF202008);山东省科技发展计划 ( 2008GG2NS02022);山东省现代农业产业技术体系中草药产业创新团队建设项目( 2016)

Comparative study on the active constituents of Salvia miltiorrhiza in different strains

LIANG Cong-lian, ZHANG Yong-qing, LI Jia, LIU Qian, PU Gao-bin*   

  1. Shandong University of Traditional Chinese Medicine, Jinan 250355,China
  • Received:2017-11-10 Online:2018-08-20 Published:2018-08-20

摘要:

为比较不同株系之间丹参有效成分的差异,采用高效液相色谱法测定丹参中包括丹参酮ⅡA在内的4种脂溶性成分和包括丹酚酸B在内的3种水溶性成分的含量,并应用SPSS软件对所得数据进行分析。通过对两大类成分的比较,发现10种株系丹参中,3、7号株系的脂溶性成分含量较高,可考虑培育为脂溶性成分提取原料;而1、9、10号株系的水溶性成分较高,可考虑培育为水溶性成分提取原料。显著性分析结果表明,所测10种不同株系丹参在其主要水溶性和脂溶性成分上存在显著差异。该研究可以为丹参优良种质的筛选提供依据。

关键词: 丹参, 比较, 有效成分, 品系选育

Abstract:

To compare the differences of effective components of Salvia miltiorrhiza from different strains, the contents of 4 fat-soluble components including tanshinone IIA and the contents of 3 water-soluble components including salvianolic acid B were measured by HPLC, and the data were analyzed by SPSS software. By comparison of the two major kinds of ingredients, it was found that the contents of fatsoluble components in 3, 7 strains were relatively higher, and the contents of watersoluble components in 1, 9, 10 strains were higher, so we could consider to cultivate 3,7 strains for the raw materials of the extraction of fatsoluble component, while cultivating 1,9,10 strains for the extraction of watersoluble components. The significance analysis results showed that there were significant differences in the main watersoluble and fatsoluble components of 10 different strains of S. miltiorrhiza. This study can provide a basis for the selection of good germplasm of S. miltiorrhiza.

Key words: active ingredients, strains breeding, comparative study, salvia miltiorrhiza Bunge.

中图分类号: 

  • R284.1