关键词: 保护作用, 丹参, 脂溶性成分, 缺氧缺糖损伤, 差异

Abstract: We compared content differences of liposoluble components of Salvia miltiorrhiza Bge.from different origin places with HPLC spectrogram.We determined the survival rate of cardiac myocytes injured by oxygen/glucose deprivation (OGD）for 1,2 and 3 h by MTT assay to construct the model group of oxygen/glucose deprivation. We further added low,medium and high dosage of liposoluble components in the cells of the model group and determined the concentration of drugs adding through the observation of cellular morphology.We then detected LDH content, TAC and survival rate (SR) of cells of every group, which were served as the parameters identifying the differences of protective effect of liposoluble components of Salvia miltiorrhiza Bge.Results show that the survival rates, LDH and TAC of OGD group and treatment group are significantly different from those of control group (P＜0.05).The survival rate, LDH and TAC of treatment group is also significantly different from those of OGD group(P＜0.05).Liposoluble components of Salvia miltiorrhiza Bge.dramatically reduce the overflow of LDH after H9C2 cardiac myocytes were injured through oxygen/glucose deprivation.They can also increase SR and TAC of cardiac myocytes,which do have protective effect to OGD damaged cardiac myocytes.Moreover, the protective effects of liposoluble components of Salvia miltiorrhiza Bge.from different origin places to H9C2 cardiac myocytes injured by OGD are indeed different.

Key words: oxygen/glucose deprivation(OGD) injury;difference, liposoluble components, Salvia miltiorrhiza Bge., protective effect