J4 ›› 2012, Vol. 25 ›› Issue (3): 75-79.doi: 10.3976/j.issn.1002-4026.2012.03.016

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血样预处理与酶电极法分析

史建国1,刘金玉2,孙士青1,张娟3   

  1. 1.山东省科学院生物研究所,山东省生物传感器重点实验室,山东 济南 250014;
    2.中国人民解放军456医院药械科,山东 济南 250031;
    3. 山东省立医院保健神经科,山东 济南 250021
  • 收稿日期:2012-02-29 出版日期:2012-06-20 发布日期:2012-06-20
  • 作者简介:史建国(1960-),男,博士,研究员,研究方向为生物传感器技术。

Pretreatment of blood specimens and their enzyme electrode method  analysis

 SHI Jian-Guo1, LIU Jin-Yu2, SUN Shi-Qing1, ZHANG Juan3   

  1. 1.Shandong Provincial Key Laboratory of Biosensors, Institute of Biology, Shandong Academy of Sciences, Jinan 250014, China; 2.Department of Pharmacy and Instrument, 456 Hospital of PLA, Jinan 250031, China; 3.Care in Department of Neurology, Shandong Provincial Hospital, Jinan 250021, China
  • Received:2012-02-29 Online:2012-06-20 Published:2012-06-20

摘要:

     采用酶电极快速测定全血血糖、血乳酸的方法评价不同条件下氟化钠、甘油醛对血糖、血乳酸的保护作用,结果表明作为血糖测定的保护剂甘油醛优于氟化钠,但是甘油醛不能用于血乳酸的保护;氟化钠作为血乳酸的保护剂,应同时采用4 ℃低温保存,才能达到最佳效果。

关键词: 葡萄糖, 乳酸, 甘油醛, 氟化钠, 酶电极

Abstract:

      We employed enzyme electrode to assess the effect of glyceraldehydes and sodium fluoride on the preservation of glucose and lactic acid in blood specimens. Experimental results show that glyceraldehydes is better than sodium fluoride in the preservation of glucose contents. However, it can not serve as the inhibition regent of lactic acid. The lactic acid remains almost constant as treated with sodium fluoride at 4 ℃.

Key words: glucose, lactic acid, glyceraldehydes, sodium fluoyide, enzyme electrode

中图分类号: 

  • R446.11