淀粉制糖过程中关键生化参数的快速测定

doi:10.3976/j.issn.1002-4026.2019.05.013

山东科学 ›› 2019, Vol. 32 ›› Issue (5): 104-109.doi: 10.3976/j.issn.1002-4026.2019.05.013

淀粉制糖过程中关键生化参数的快速测定

杨俊慧¹，马恒¹，马耀宏¹，赵玉斌²，孙慧囡¹，史建国^1*

1.齐鲁工业大学（山东省科学院），山东省科学院生物研究所，山东省生物传感器重点实验室，山东济南 250014;2.鲁洲生物科技（山东）有限公司，山东临沂 276000

收稿日期:2019-05-20 出版日期:2019-10-20 发布日期:2019-10-09
通信作者: 史建国（1960—），男，研究员，研究方向为微生物学。E-mail: shijg@sdas.org
作者简介:杨俊慧(1969—)，女，高级实验师，研究方向为生物学。E-mail: swdz@sdas.org
基金资助:
山东省重点研发计划（公益类）（2017GSF221014）

Rapid analysis of key biochemical parameters during starch sugar production

YANG Jun-hui¹, MA Heng¹, MA Yao-hong¹, ZHAO Yu-bin², SUN Hui-nan¹, SHI Jian-guo^1*

1.Shandong Provincial Key Laboratory of Biosensors,Biology Institute, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250014, China; 2. Luzhou Biotechnology (Shandong) Co., Ltd., Linyi 276000, China

Received:2019-05-20 Online:2019-10-20 Published:2019-10-09

摘要/Abstract

摘要： 建立了葡萄糖、还原糖、DE（dextrose equivalent)值等淀粉制糖过程中关键参数的快速分析方法，对淀粉制糖过程中的关键参数进行了快速测定,检测淀粉制糖过程中葡萄糖、还原糖、DE值等关键参数的变化规律。发现液化过程可以明显增加葡萄糖含量，降低麦芽三糖、麦芽四糖含量；糖化过程中随着时间延长，葡萄糖、还原糖含量逐渐增加，DE值升高。该方法可以为淀粉制糖过程控制与分析提供新的技术支持。

关键词: 淀粉制糖, 快速测定；生化参数；葡萄糖；DE值

Abstract: In this study, a rapid analytical method was developed for such key parameters as glucose, reducing sugar, and dextrose equivalent (DE) during starch sugar production. The developed method was applied to rapidly determine the key parameters during starch sugar production. The variation tendency of these key parameters (glucose, reducing sugar, and DE) was examined. The results indicated that liquefaction significantly increased glucose levels but decreased maltotriose and maltotetraose levels. During saccharification, glucose and reducing sugar levels increased with time, and DE was elevated. The developed rapid analysis method for key parameters during starch sugar production can provide new technical support for the quality control and analysis of starch sugar production.

Key words: starch sugar production, rapid analysis, biochemical parameters, glucose, DE

中图分类号:

TS245.4

杨俊慧, 马恒, 马耀宏, 赵玉斌, 孙慧囡, 史建国. 淀粉制糖过程中关键生化参数的快速测定[J]. 山东科学, 2019, 32(5): 104-109.

YANG Jun-hui, MA Heng, MA Yao-hong, ZHAO Yu-bin, SUN Hui-nan, SHI Jian-guo. Rapid analysis of key biochemical parameters during starch sugar production[J]. Shandong Science, 2019, 32(5): 104-109.

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淀粉制糖过程中关键生化参数的快速测定

Rapid analysis of key biochemical parameters during starch sugar production

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