电流型酶电极生物传感器抗可逆抑制剂干扰的测定方法

doi:10.3976/j.issn.1002-4026.2021.04.014

山东科学 ›› 2021, Vol. 34 ›› Issue (4): 87-94.doi: 10.3976/j.issn.1002-4026.2021.04.014

电流型酶电极生物传感器抗可逆抑制剂干扰的测定方法

朱思荣，毕春元，杜祎，张金玲，高广恒，张利群，赵晓华，杨艳

齐鲁工业大学（山东省科学院）山东省科学院生物研究所山东省生物传感器重点实验室，山东济南 250103

收稿日期:2020-08-25 出版日期:2021-07-30 发布日期:2021-08-03
作者简介:朱思荣（1963—）男，硕士，研究员，研究方向为生物传感器及相关产品开发。E-mail: yanzhi@sdas.org
基金资助:
山东省重点研发计划（2018GSF121023，2019GSF107017）；山东省科学院院地产学研协同创新基金（2018CXY-12）

An analysis method for suppressing interference from the reversible inhibitors of amperometric enzyme electrode biosensors

ZHU Si-rong, BI Chun-yuan, DU Yi, ZHANG Jin-ling, GAO Guang-heng,ZHANG Li-qun, ZHAO Xiao-hua, YANG Yan

Shandong Provincial Key Laboratory of Biosensor, Biology Institute, Qilu University of Technology (Shandong Academy of sciences), Jinan 250103，China

Received:2020-08-25 Online:2021-07-30 Published:2021-08-03

摘要/Abstract

摘要： 电流型酶电极生物传感器在工业领域的应用中经常会遇到酶活性抑制剂干扰问题，在存在酶活性抑制剂的环境下，用酶电极生物传感检测，分析结果会显著偏低。根据电流型酶电极生物传感器的检测原理，设计了一种抗抑制剂干扰的测定方法，传感器正常定标完成后，先按正常方法进行测定，然后用样品加标样进行第二次测定，两次测定具有相同的酶抑制剂环境。通过两次测定结果比较，算出第二次测定所加标样的响应结果，计算出抑制剂对酶活的抑制系数，最终通过第一次测定结果和抑制系数计算出样品中待测物的浓度。这种测定方法能有效消除酶抑制剂对分析结果的影响，拓展酶电极生物传感器在工业领域中的应用范围。

关键词: 酶电极, 生物传感器, 酶抑制剂, 抗干扰, 测定方法

Abstract: Enzyme inhibitor interference problems are often encountered when amperometric enzyme electrode biosensors are used in industrial fields. Test results of enzyme electrode biosensing detection tend to be significantly lower if enzyme activity inhibitors are present in samples. According to the detection principle of amperometric enzyme electrode biosensors, a testing method was designed to counter the interference of inhibitors. After the calibration of the biosensor, a single sample was first tested and then the same sample was added with a known standard sample and tested for the second time. The two tests were performed under the same conditions of enzyme inhibitors. Through a comparison of the results of two tests, the inhibition coefficient was calculated according to the determined response of the added standard sample at the second test.Finally, the unknown sample was calculated based on the result of the first test and inhibition coefficient. This analysis method can effectively eliminate the influence of enzyme inhibitors on the analysis and expand the application range of biosensors in industrial fields.

Key words: enzyme electrode, biosensor, enzyme inhibitor, interference suppression, determination method

中图分类号:

TP 212.3

朱思荣, 毕春元, 杜祎, 张金玲, 高广恒, 张利群, 赵晓华, 杨艳. 电流型酶电极生物传感器抗可逆抑制剂干扰的测定方法[J]. 山东科学, 2021, 34(4): 87-94.

ZHU Si-rong, BI Chun-yuan, DU Yi, ZHANG Jin-ling, GAO Guang-heng, ZHANG Li-qun, ZHAO Xiao-hua, YANG Yan. An analysis method for suppressing interference from the reversible inhibitors of amperometric enzyme electrode biosensors[J]. Shandong Science, 2021, 34(4): 87-94.

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电流型酶电极生物传感器抗可逆抑制剂干扰的测定方法

An analysis method for suppressing interference from the reversible inhibitors of amperometric enzyme electrode biosensors

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