果汁中乙醇质量浓度的抗干扰检测方法

doi:10.3976/j.issn.1002-4026.2022.04.011

山东科学 ›› 2022, Vol. 35 ›› Issue (4): 84-89.doi: 10.3976/j.issn.1002-4026.2022.04.011

果汁中乙醇质量浓度的抗干扰检测方法

王丙莲, 马耀宏(), 公维丽, 杨艳, 郑岚, 刘庆艾, 孟庆军

齐鲁工业大学(山东省科学院) 山东省科学院生物研究所,山东济南 250103

收稿日期:2021-03-29 出版日期:2022-08-20 发布日期:2022-07-25
通信作者: 马耀宏 E-mail:2320073664@qq.com
作者简介:王丙莲(1979—),女,硕士,副研究员,研究方向为分析检测及生物传感技术。
基金资助:
山东省自然科学基金(ZR2019BC072);山东省重点研发计划(重大科技创新工程)(2020CXGC010602);齐鲁工业大学(山东省科学院)科教产融合创新试点工程项目(2020KJC-ZD15)

Anti-interference detection method of ethanol concentrationin fruit juices

WANG Bing-lian, MA Yao-hong(), GONG Wei-li, YANG Yan, ZHENG Lan, LIU Qing-ai, MENG Qing-jun

Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China

Received:2021-03-29 Online:2022-08-20 Published:2022-07-25
Contact: MA Yao-hong E-mail:2320073664@qq.com

摘要/Abstract

摘要：

针对生物传感器法测定果汁中乙醇质量浓度易受电化学活性物质干扰的问题,通过制备乙醇辅助酶电极,使工作酶电极和辅助酶电极协同工作,构建了一种新型的乙醇检测系统,实现了乙醇含量快速准确的检测。结果表明,该检测系统用于果汁乙醇质量浓度检测时,稳定性好、结果准确,抗干扰能力强。重复测定的相对标准偏差为0.80%;加标回收率为99.10%~101.10 %;葡萄糖、蔗糖、柠檬酸、Ca²⁺、过氧化氢酶以及电化学活性物质维生素C等干扰物存在的情况下,乙醇测定结果不受影响;与国标法、气相色谱法相比较,差异不明显(P>0.05)。该方法操作简单、成本低廉且准确度高,能够满足低成本要求的实际生产要求。

关键词: 果汁, 乙醇检测, 辅助酶电极, 抗干扰

Abstract:

To eliminate the influence of electrochemically active compounds on ethanol determination in fruit juices via biosensor, an ethanol-assisted enzyme electrode was prepared and a novel alcohol biological detection system was constructed using the synergy of working and auxiliary enzyme electrodes. The anti-interference property of the system was tested and the anti-interference coefficient was fitted. Thereafter, the calculation formula of ethanol concentration was deduced to achieve the rapid and accurate detection of ethanol concentration. The results showed that the anti-interference performance of the detection was stable with good stability, accurate results, and strong ability. When the method was applied to determine the ethanol concentration in fruit juices, the relativity standard division was 0.80% and the recoveries were 99.10% to 101.10%. Glucose, sucrose, citric acid, Ca²⁺, catalase, and vatamine C in the fruit juices had no effect on ethanol detection. T-test analysis showed that there was no significant difference between this method and the national standard and gas chromatography (P>0.05).The method is simple in operation,low in cost and high in accuracy,which can meet the production requirements of low cost.

Key words: fruit juice, ethanol detection, auxiliary enzyme electrode, anti-interference

中图分类号:

TS275.5

王丙莲, 马耀宏, 公维丽, 杨艳, 郑岚, 刘庆艾, 孟庆军. 果汁中乙醇质量浓度的抗干扰检测方法[J]. 山东科学, 2022, 35(4): 84-89.

WANG Bing-lian, MA Yao-hong, GONG Wei-li, YANG Yan, ZHENG Lan, LIU Qing-ai, MENG Qing-jun. Anti-interference detection method of ethanol concentrationin fruit juices[J]. Shandong Science, 2022, 35(4): 84-89.

图/表 5

表1

抗干扰性能参数"

项目	性能参数数值
工作酶电极相对电信号(A₁)	52	50	51
辅助酶电极相对电信号(A₀)	1 648	1 587	1 604
A₁/A₀	0.031 6	0.031 5	0.031 8
Δ(A₁/A₀)		0.316%	0.633%
$A 1 / A 0 ¯$	0.031 6

表1

表2

系统定标数据"

项目	测定结果(n = 2)
工作酶电极电信号(S₁)	2 525	2 497
辅助酶电极电信号(S₀)	208	52
K₄Fe(CN)₆抗干扰初测试均值( $A 1 / A 0 ¯$ )	0.031 6
工作电极酶活性(I₁)	2 518
工作电极酶活性(I₂)	2 495
工作酶电极活性变化率(ΔI/I₁)	0.92%
工作电极酶活性平均值( $I -$ )	2 506

表2

表3

表4

表5

参考文献 11

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测定次数	工作酶电极电信号X	辅助酶电极电信号A_样	乙醇质量浓度/(mg·mL^-1)
1	1 298	63	9.910
2	1 295	57	9.930
3	1 369	125	1.003
4	1 348	118	9.910
5	1 336	77	1.011
平均值	1 329	88	9.980
相对标准偏差	2.18%	31.82%	0.80%

样品	乙醇质量浓度/(mg·mL^-1)				相对标准偏差/%	回收率/%
样品	1	2	3	4	相对标准偏差/%	回收率/%
对照样品	3.540	3.610	3.620	3.550	0.99	99.10~101.10
加标样品	1.345	1.368	1.372	1.362	0.88

测定次数	乙醇质量浓度/(mg·mL^-1)
	乙醇检测系统法		国标法		GC法
	1^#	2^#	1^#	2^#	1^#	2^#
1	2.15	2.86	2.21	2.81	2.17	2.85
2	2.13	2.82	2.13	2.92	2.18	2.90
3	2.16	2.83	2.17	2.80	2.14	2.86
4	2.14	2.87	2.25	2.95	2.19	2.91
平均值	2.145	2.845	2.19	2.87	2.17	2.88
相对标准偏差/%	0.69	0.72	1.83	2.29	0.40	0.88
T检验	—	—	0.14	0.55	0.09	0.11

果汁中乙醇质量浓度的抗干扰检测方法

Anti-interference detection method of ethanol concentrationin fruit juices

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